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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
5
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pubmed:dateCreated |
1990-9-20
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pubmed:abstractText |
Identification of tissue-specific DNaseI hypersensitive sites in the TCR J beta 2-C beta 2 intron has suggested the presence of sequences involved in the regulation of gene expression. Therefore, we have searched for protein-DNA interactions within a 930-bp fragment derived from the J beta 2-C beta 2 intron by in vitro DNaseI protection experiments and electrophoretic mobility-shift assays. This analysis has revealed, in addition to a previously characterized NF-kappa B binding site, the presence of seven potential protein-DNA interaction sites within this fragment. Interestingly, they are clustered in the regions where in vivo T cell-specific nuclease hypersensitive sites have been previously identified. Binding sites for four potential transcription factors have been mapped precisely by methylation-interference experiments. Sequence comparisons show that one of them is homologous to the Y box present in the promoter regions of MHC class II genes. Identification of several protein-DNA interactions clustered within the J beta 2-C beta 2 intron and the presence of binding sites for two well-characterized transcription factors suggest a transcriptional regulatory function for this region.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Oligonucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Antigen, T-Cell,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Antigen, T-Cell...
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0022-1767
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
145
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1577-82
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:2143520-Animals,
pubmed-meshheading:2143520-Base Sequence,
pubmed-meshheading:2143520-Cell Line,
pubmed-meshheading:2143520-DNA-Binding Proteins,
pubmed-meshheading:2143520-Enhancer Elements, Genetic,
pubmed-meshheading:2143520-Gene Expression Regulation,
pubmed-meshheading:2143520-Genes,
pubmed-meshheading:2143520-Introns,
pubmed-meshheading:2143520-Mice,
pubmed-meshheading:2143520-Molecular Sequence Data,
pubmed-meshheading:2143520-Nuclear Proteins,
pubmed-meshheading:2143520-Oligonucleotides,
pubmed-meshheading:2143520-Receptors, Antigen, T-Cell,
pubmed-meshheading:2143520-Receptors, Antigen, T-Cell, alpha-beta,
pubmed-meshheading:2143520-Restriction Mapping
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pubmed:year |
1990
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pubmed:articleTitle |
Multiple nuclear factors interact with sequences within the J beta 2-C beta 2 intron of the murine T cell receptor beta-chain gene.
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pubmed:affiliation |
Department of Biology, Brandeis University, Waltham, MA 02254-9110.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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