Linked Life Data

21435193

Source:http://linkedlifedata.com/resource/pubmed/id/21435193

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2011-5-17
pubmed:abstractText
To identify microRNAs potentially involved in melanomagenesis, we compared microRNA expression profiles between melanoma cell lines and cultured melanocytes. The most differentially expressed microRNA between the normal and tumor cell lines was miR-211. We focused on this pigment-cell-enriched miRNA as it is derived from the microphthalmia-associated transcription factor (MITF)-regulated gene, TRPM1 (melastatin). We find that miR-211 expression is greatly decreased in melanoma cells and melanoblasts compared to melanocytes. Bioinformatic analysis identified a large number of potential targets of miR-211, including POU3F2 (BRN2). Inhibition of miR-211 in normal melanocytes resulted in increased BRN2 protein, indicating that endogenous miR-211 represses BRN2 in differentiated cells. Over-expression of miR-211 in melanoma cell lines changed the invasive potential of the cells in vitro through directly targeting BRN2 translation. We propose a model for the apparent non-overlapping expression levels of BRN2 and MITF in melanoma, mediated by miR-211 expression.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1755-148X
pubmed:author
pubmed:copyrightInfo
2011 John Wiley & Sons A/S.
pubmed:issnType
Electronic
pubmed:volume
24
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
525-37
pubmed:meshHeading
pubmed:year
2011
pubmed:articleTitle
Melanoma cell invasiveness is regulated by miR-211 suppression of the BRN2 transcription factor.
pubmed:affiliation
Drug Discovery Group, Division of Cancer & Cell Biology, Queensland Institute of Medical Research, Brisbane, QLD, Australia. Glen.Boyle@qimr.edu.au ?
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't