Linked Life Data

21431508

Source:http://linkedlifedata.com/resource/pubmed/id/21431508

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2011-3-24
pubmed:abstractText
Human mesenchymal stromal cells (MSC) raise high hopes for tissue engineering and therapeutic -applications. So far, it is not possible to isolate pure fractions from bone marrow and therefore MSC cell preparations notoriously represent heterogeneous mixtures of different cell types. The composition of -subpopulations can already be affected by the initial steps of cell preparation. Usually, isolation of MSC involves density fractionation to separate the mononuclear cells (MNCs) from erythrocytes and -granulocytes. However, this method is difficult to standardize especially under GMP conditions. Here, we describe an alternative approach for isolation of human MSC based on red blood cell (RBC) lysis with ammonium chloride. This results in a slightly higher number of fibroblastic colony forming units (CFU-F), whereas morphological analysis of the CFU-F reveals the same heterogeneous composition of MSC cultures indicating that the proportion of subpopulations is not affected by RBC lysis. Immunophenotype (CD73+, CD90+, CD105+, CD31-, CD34-, CD45-), adipogenic, and osteogenic differentiation potential of MSC were also similar with both methods. In conclusion, RBC lysis comprises an efficient method for the isolation of human MSC from bone marrow aspirate. This technique is faster and can be standardized more easily for clinical application of MSC.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1940-6029
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
698
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
23-35
pubmed:meshHeading
pubmed:year
2011
pubmed:articleTitle
Standardized isolation of human mesenchymal stromal cells with red blood cell lysis.
pubmed:affiliation
Helmholtz Institute for Biomedical Engineering, Department of Cell Biology, RWTH Aachen University Medical School, Pauwelsstrasse 20, 52074 Aachen, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't