Source:http://linkedlifedata.com/resource/pubmed/id/21430102
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
5
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pubmed:dateCreated |
2011-4-28
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pubmed:abstractText |
PCR-hybridization was compared to culture methods for evaluating suspected blood infections. A total of 231 clinical samples from blood culture bottles that were flagged positive by the BacT/Alert system or were negative 1 week after inoculation were tested. When the PCR-hybridization and culture method results were compared, the positive and negative concordance rates were 99.2% (122/123) and 89.5% (94/105), respectively. Of the negative blood cultures, 10.5% (11/105) were positive by PCR-hybridization. Supplemental testing of negative blood cultures may identify bacterial pathogens that are undetectable by culture methods.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
1098-660X
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pubmed:author | |
pubmed:issnType |
Electronic
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pubmed:volume |
49
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2031-4
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pubmed:dateRevised |
2011-11-1
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pubmed:meshHeading |
pubmed-meshheading:21430102-Adolescent,
pubmed-meshheading:21430102-Bacteremia,
pubmed-meshheading:21430102-Bacteria,
pubmed-meshheading:21430102-Bacteriological Techniques,
pubmed-meshheading:21430102-Child,
pubmed-meshheading:21430102-Child, Preschool,
pubmed-meshheading:21430102-DNA, Bacterial,
pubmed-meshheading:21430102-DNA, Ribosomal,
pubmed-meshheading:21430102-Humans,
pubmed-meshheading:21430102-Infant,
pubmed-meshheading:21430102-Infant, Newborn,
pubmed-meshheading:21430102-Nucleic Acid Hybridization,
pubmed-meshheading:21430102-Polymerase Chain Reaction,
pubmed-meshheading:21430102-RNA, Ribosomal, 16S,
pubmed-meshheading:21430102-Sensitivity and Specificity
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pubmed:year |
2011
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pubmed:articleTitle |
Bacterial identification by 16S rRNA gene PCR-hybridization as a supplement to negative culture results.
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pubmed:affiliation |
Division of Infectious Disease, Children's Hospital Los Angeles, and Metic Transplantation Laboratory,Keck School of Medicine, University of Southern California, Los Angeles, California, USA. kmatsuda@usc.edu.
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pubmed:publicationType |
Journal Article,
Evaluation Studies
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