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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
19
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pubmed:dateCreated |
2011-5-2
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pubmed:abstractText |
CD47 is a transmembrane protein that is a marker of "self". CD47 binding to its cognate receptor in leukocytes and macrophages, signal-regulatory protein alpha (SIRP?), causes inhibition of inflammatory cell attachment. We hypothesized that immobilization of recombinant CD47 on polymeric surfaces would reduce inflammation. Recombinant CD47 was appended to polyvinyl chloride (PVC) or polyurethane (PU) surfaces via photoactivation chemistry. Cell culture studies showed that CD47 immobilization significantly reduced human neutrophil (HL-60) and human monocyte derived macrophage (MDM) (THP-1) attachment to PVC and PU respectively. A neutralizing antibody, directed against SIRP?, inhibited THP-1 and HL-60 binding to PU and PVC surfaces respectively. This antibody also increased the level of SIRP? tyrosine phosphorylation, thereby indicating a direct role for SIRP? mediated signaling in preventing inflammatory cell attachment. Studies using human blood in an ex vivo flow-loop showed that CD47 modified PVC tubing significantly reduced cell binding and neutrophil activation compared to unmodified tubing or poly-2-methoxy-ethylacrylate (PMEA) coated tubing. In ten-week rat subdermal implants, CD47 functionalized PU films showed a significant reduction in markers of MDM mediated oxidative degradation compared to unmodified PU. In conclusion, CD47 functionalized surfaces can resist inflammatory cell interactions both in vitro and in vivo.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD47,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Differentiation,
http://linkedlifedata.com/resource/pubmed/chemical/Biocompatible Materials,
http://linkedlifedata.com/resource/pubmed/chemical/Polymers,
http://linkedlifedata.com/resource/pubmed/chemical/Polyurethanes,
http://linkedlifedata.com/resource/pubmed/chemical/Polyvinyl Chloride,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Immunologic,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/SIRPA protein, human
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
1878-5905
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pubmed:author |
pubmed-author:AlferievIvan SIS,
pubmed-author:ConnollyJeanne MJM,
pubmed-author:DischerDennis EDE,
pubmed-author:EckellsEdward CEC,
pubmed-author:EckmannDavid MDM,
pubmed-author:FinleyMatthew JMJ,
pubmed-author:LevyRobert JRJ,
pubmed-author:StachelekStanley JSJ,
pubmed-author:TomczykNancyN,
pubmed-author:TsaiRichard KRK,
pubmed-author:WangFengxiangF
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pubmed:copyrightInfo |
Copyright © 2011 Elsevier Ltd. All rights reserved.
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pubmed:issnType |
Electronic
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pubmed:volume |
32
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4317-26
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pubmed:dateRevised |
2011-9-26
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pubmed:meshHeading |
pubmed-meshheading:21429575-Animals,
pubmed-meshheading:21429575-Antigens, CD47,
pubmed-meshheading:21429575-Antigens, Differentiation,
pubmed-meshheading:21429575-Biocompatible Materials,
pubmed-meshheading:21429575-Cell Adhesion,
pubmed-meshheading:21429575-HL-60 Cells,
pubmed-meshheading:21429575-Humans,
pubmed-meshheading:21429575-Implants, Experimental,
pubmed-meshheading:21429575-Inflammation,
pubmed-meshheading:21429575-Macrophages,
pubmed-meshheading:21429575-Male,
pubmed-meshheading:21429575-Materials Testing,
pubmed-meshheading:21429575-Molecular Structure,
pubmed-meshheading:21429575-Photochemistry,
pubmed-meshheading:21429575-Polymers,
pubmed-meshheading:21429575-Polyurethanes,
pubmed-meshheading:21429575-Polyvinyl Chloride,
pubmed-meshheading:21429575-Rats,
pubmed-meshheading:21429575-Rats, Sprague-Dawley,
pubmed-meshheading:21429575-Receptors, Immunologic,
pubmed-meshheading:21429575-Recombinant Proteins,
pubmed-meshheading:21429575-Surface Properties
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pubmed:year |
2011
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pubmed:articleTitle |
The effect of CD47 modified polymer surfaces on inflammatory cell attachment and activation.
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pubmed:affiliation |
Division of Cardiology, Department of Pediatrics, The Children's Hospital of Philadelphia, PA, USA. stachelek@email.chop.edu
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Evaluation Studies,
Research Support, N.I.H., Extramural
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