Source:http://linkedlifedata.com/resource/pubmed/id/21425409
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
2011-3-22
|
| pubmed:abstractText |
We have used homologous recombination in human embryonic stem cells (hESCs) to insert sequences encoding green fluorescent protein (GFP) into the NKX2.1 locus, a gene required for normal development of the basal forebrain. Generation of NKX2.1-GFP(+) cells was dependent on the concentration, timing, and duration of retinoic acid treatment during differentiation. NKX2.1-GFP(+) progenitors expressed genes characteristic of the basal forebrain, including SHH, DLX1, LHX6, and OLIG2. Time course analysis revealed that NKX2.1-GFP(+) cells could upregulate FOXG1 expression, implying the existence of a novel pathway for the generation of telencephalic neural derivatives. Further maturation of NKX2.1-GFP(+) cells gave rise to ?-aminobutyric acid-, tyrosine hydroxylase-, and somatostatin-expressing neurons as well as to platelet-derived growth factor receptor ?-positive oligodendrocyte precursors. These studies highlight the diversity of cell types that can be generated from human NKX2.1(+) progenitors and demonstrate the utility of NKX2.1(GFP/w) hESCs for investigating human forebrain development and neuronal differentiation.
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| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Mar
|
| pubmed:issn |
1549-4918
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| pubmed:author |
pubmed-author:AldenDarymD,
pubmed-author:AndersonStewart ASA,
pubmed-author:BourkeJustinJ,
pubmed-author:DavisRichard PRP,
pubmed-author:ElefantyAndrew GAG,
pubmed-author:ElliottDavid ADA,
pubmed-author:GiudiceAntoniettaA,
pubmed-author:GoulburnAdam LAL,
pubmed-author:HatzistavrouTanyaT,
pubmed-author:HaynesJohn MJM,
pubmed-author:LangRichard JRJ,
pubmed-author:LimSue MeiSM,
pubmed-author:MicallefSuzanne JSJ,
pubmed-author:NgElizabeth SES,
pubmed-author:PoutonColin WCW,
pubmed-author:SohChew-LiCL,
pubmed-author:StanleyEdouard GEG,
pubmed-author:TrounsonAlan OAO,
pubmed-author:WatmuffBradleyB,
pubmed-author:YuQing CQC
|
| pubmed:copyrightInfo |
Copyright © 2011 AlphaMed Press.
|
| pubmed:issnType |
Electronic
|
| pubmed:volume |
29
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
462-73
|
| pubmed:meshHeading |
pubmed-meshheading:21425409-Animals,
pubmed-meshheading:21425409-Animals, Newborn,
pubmed-meshheading:21425409-Cell Differentiation,
pubmed-meshheading:21425409-Cell Lineage,
pubmed-meshheading:21425409-Cell Tracking,
pubmed-meshheading:21425409-Cells, Cultured,
pubmed-meshheading:21425409-Embryonic Stem Cells,
pubmed-meshheading:21425409-Flow Cytometry,
pubmed-meshheading:21425409-Genes, Reporter,
pubmed-meshheading:21425409-Humans,
pubmed-meshheading:21425409-Mice,
pubmed-meshheading:21425409-Mice, Transgenic,
pubmed-meshheading:21425409-Molecular Targeted Therapy,
pubmed-meshheading:21425409-Neurogenesis,
pubmed-meshheading:21425409-Nuclear Proteins,
pubmed-meshheading:21425409-Prosencephalon,
pubmed-meshheading:21425409-Transcription Factors
|
| pubmed:year |
2011
|
| pubmed:articleTitle |
A targeted NKX2.1 human embryonic stem cell reporter line enables identification of human basal forebrain derivatives.
|
| pubmed:affiliation |
Monash Immunology and Stem Cell Laboratories, Monash University, Clayton, Victoria, Australia.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Evaluation Studies
|