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rdf:type |
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lifeskim:mentions |
umls-concept:C0007634,
umls-concept:C0013935,
umls-concept:C0016030,
umls-concept:C0017262,
umls-concept:C0029016,
umls-concept:C0031437,
umls-concept:C0032136,
umls-concept:C0034693,
umls-concept:C0034721,
umls-concept:C0185117,
umls-concept:C1510411,
umls-concept:C1521970,
umls-concept:C1522602,
umls-concept:C2826170,
umls-concept:C2911684
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|
pubmed:issue |
2
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|
pubmed:dateCreated |
1990-8-13
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|
pubmed:abstractText |
Rat embryonal fibroblasts of the second passage have been immortalized or transformed with the E1A region of the human adenovirus type 5 and the cloned c-Ha-ras oncogene. Several cell lines obtained greatly differ according to the criteria of the transformed phenotype: saturation cell density, the ability to be cloned on a substrate and in soft agar, the ability to give tumors in nude mice. We have transiently transfected these immortalized and transformed cell lines with CAT-plasmids containing the CAT gene under the control of promoters of the "cell-cycle dependent" genes (human hsp70 and protooncogene c-fos). The results showed that the expression of hsp-CAT and fos-CAT plasmids differed in great extent depending on whether the cells were immortalized (E1Aad5) or transformed (E1Aad5+Ha-ras). The role of cellular genes and cellular transcription factors influencing the expression of transiently transfected CAT-plasmids has been discussed.
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pubmed:language |
rus
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pubmed:journal |
|
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
0041-3771
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pubmed:author |
|
|
pubmed:issnType |
Print
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pubmed:volume |
32
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
148-55
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:2142348-Animals,
pubmed-meshheading:2142348-Cell Line,
pubmed-meshheading:2142348-Cell Transformation, Neoplastic,
pubmed-meshheading:2142348-Cell Transformation, Viral,
pubmed-meshheading:2142348-Cells, Cultured,
pubmed-meshheading:2142348-Embryo, Mammalian,
pubmed-meshheading:2142348-Fibroblasts,
pubmed-meshheading:2142348-Gene Expression,
pubmed-meshheading:2142348-Genes, ras,
pubmed-meshheading:2142348-Mice,
pubmed-meshheading:2142348-Mice, Nude,
pubmed-meshheading:2142348-Neoplasm Transplantation,
pubmed-meshheading:2142348-Oncogene Proteins,
pubmed-meshheading:2142348-Oncogenes,
pubmed-meshheading:2142348-Phenotype,
pubmed-meshheading:2142348-Plasmids,
pubmed-meshheading:2142348-Rats,
pubmed-meshheading:2142348-Rats, Inbred Strains,
pubmed-meshheading:2142348-Transcriptional Activation,
pubmed-meshheading:2142348-Transfection
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pubmed:year |
1990
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|
pubmed:articleTitle |
[The characteristics of the transformed phenotype and the expression of indicator plasmids in the cells of rat embryonic fibroblasts immortalized by oncogene E1Aad5 and transformed by oncogenes E1Aad5+c-Ha-ras].
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pubmed:publicationType |
Journal Article,
Comparative Study,
English Abstract
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