Linked Life Data

21420399

Source:http://linkedlifedata.com/resource/pubmed/id/21420399

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2011-4-25
pubmed:abstractText
Mammalian tooth development depends on activation of odontogenic potential in the presumptive dental mesenchyme by the Msx1 and Pax9 transcription factors. We recently reported that the zinc finger transcription factor Osr2 was expressed in a lingual-to-buccal gradient pattern surrounding the developing mouse molar tooth germs and mice lacking Osr2 developed supernumerary teeth lingual to their molars. We report here generation of a gene-targeted mouse strain that allows conditional inactivation of Pax9 and subsequent activation of expression of Osr2 in the developing tooth mesenchyme from the Pax9 locus. Expression of Osr2 from one copy of the Pax9 gene did not disrupt normal tooth development but was sufficient to suppress supernumerary tooth formation in the Osr2(-/-) mutant mice. We found that endogenous Osr2 mRNA expression was significantly downregulated in the developing tooth mesenchyme in Pax9(del/del) mice. Mice lacking both Osr2 and Pax9 exhibited early tooth developmental arrest with significantly reduced Bmp4 and Msx1 mRNA expression in the developing tooth mesenchyme, similar to that in Pax9(del/del) mutants but in contrast to the rescue of tooth morphogenesis in Msx1(-/-)Osr2(-/-) double mutant mice. Furthermore, we found that Osr2 formed stable protein complexes with the Msx1 protein and interacted weakly with the Pax9 protein in co-transfected cells. These data indicate that Osr2 acts downstream of Pax9 and patterns the mesenchymal odontogenic field through protein-protein interactions with Msx1 and Pax9 during early tooth development.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1095-564X
pubmed:author
pubmed:copyrightInfo
Copyright © 2011 Elsevier Inc. All rights reserved.
pubmed:issnType
Electronic
pubmed:day
15
pubmed:volume
353
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
344-53
pubmed:meshHeading
pubmed-meshheading:21420399-Animals, pubmed-meshheading:21420399-Base Sequence, pubmed-meshheading:21420399-Bone Morphogenetic Protein 4, pubmed-meshheading:21420399-DNA, pubmed-meshheading:21420399-Female, pubmed-meshheading:21420399-Gene Expression Regulation, Developmental, pubmed-meshheading:21420399-Gene Knockout Techniques, pubmed-meshheading:21420399-MSX1 Transcription Factor, pubmed-meshheading:21420399-Male, pubmed-meshheading:21420399-Mesoderm, pubmed-meshheading:21420399-Mice, pubmed-meshheading:21420399-Mice, 129 Strain, pubmed-meshheading:21420399-Mice, Inbred C57BL, pubmed-meshheading:21420399-Mice, Knockout, pubmed-meshheading:21420399-Mice, Transgenic, pubmed-meshheading:21420399-Odontogenesis, pubmed-meshheading:21420399-Paired Box Transcription Factors, pubmed-meshheading:21420399-Pregnancy, pubmed-meshheading:21420399-Protein Interaction Mapping, pubmed-meshheading:21420399-RNA, Messenger, pubmed-meshheading:21420399-Tooth Germ, pubmed-meshheading:21420399-Transcription Factors
pubmed:year
2011
pubmed:articleTitle
Osr2 acts downstream of Pax9 and interacts with both Msx1 and Pax9 to pattern the tooth developmental field.
pubmed:affiliation
Center for Oral Biology and Department of Biomedical Genetics, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, USA.
pubmed:publicationType
Journal Article, Research Support, N.I.H., Extramural