Linked Life Data

21419803

Source:http://linkedlifedata.com/resource/pubmed/id/21419803

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
2011-5-16
pubmed:abstractText
Yellow fever virus (YFV), a member of the family Flaviviridae, genus Flavivirus is endemic to tropical areas of Africa and South America and is among the arboviruses that pose a threat to public health. Recent outbreaks in Brazil, Bolivia, and Paraguay and the observation that vectors capable of transmitting YFV are presenting in urban areas underscore the urgency of improving surveillance and diagnostic methods. Two novel methods (RT-hemi-nested-PCR and SYBR(®) Green qRT-PCR) for efficient detection of YFV strains circulating in South America have been developed. The methods were validated using samples obtained from golden hamsters infected experimentally with wild-type YFV strains as well as human serum and tissue samples with acute disease.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1879-0984
pubmed:author
pubmed:copyrightInfo
Copyright © 2011 Elsevier B.V. All rights reserved.
pubmed:issnType
Electronic
pubmed:volume
174
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
29-34
pubmed:meshHeading
pubmed:year
2011
pubmed:articleTitle
Evaluation of two molecular methods for the detection of Yellow fever virus genome.
pubmed:affiliation
Department of Arbovirology and Hemorrhagic Fevers, Instituto Evandro Chagas, Ministry of Health, Ananindeua, Brazil. marcionunes@iec.pa.gov.br
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Validation Studies