| pubmed-article:2141629 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2141629 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:2141629 | lifeskim:mentions | umls-concept:C0312738 | lld:lifeskim |
| pubmed-article:2141629 | lifeskim:mentions | umls-concept:C0007589 | lld:lifeskim |
| pubmed-article:2141629 | lifeskim:mentions | umls-concept:C0205307 | lld:lifeskim |
| pubmed-article:2141629 | lifeskim:mentions | umls-concept:C1533691 | lld:lifeskim |
| pubmed-article:2141629 | lifeskim:mentions | umls-concept:C1511938 | lld:lifeskim |
| pubmed-article:2141629 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:2141629 | pubmed:dateCreated | 1990-8-1 | lld:pubmed |
| pubmed-article:2141629 | pubmed:abstractText | The differentiation of surface Ig- pre-B cells into surface Ig+ B cells is a critical transition in mammalian B cell ontogeny. Elucidation of the growth factor requirements and differentiative potential of human pre-B cells has been hampered by the absence of a reproducible culture system that supports differentiation. Fluorescence-activated cell sorting and magnetic bead depletion were used to purify fetal bone marrow CD10+/surface mu- cells, which contain 60-70% cytoplasmic mu+ pre-B cells. CD10+/surface mu- cells cultured for 2 d were observed to differentiate into surface mu+ cells. Analysis by Southern blotting provided direct evidence that rearrangement of kappa light chain genes occurs in culture, and flow cytometric analysis revealed the appearance of surface Ig+ B cells expressing mu/kappa or mu/lambda. Unexpectedly, the kappa/lambda ratio in differentiated cells was the inverse of what is normally observed in adult peripheral blood. Differentiation occurs in the absence of exogenous growth factors or cytokines, suggesting that a stimulus-independent differentiative inertia might characterize pre-B cells in vivo. Future use of this model will facilitate our understanding of normal and abnormal human pre-B cell differentiation. | lld:pubmed |
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| pubmed-article:2141629 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2141629 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2141629 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:2141629 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2141629 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:2141629 | pubmed:issn | 0022-1007 | lld:pubmed |
| pubmed-article:2141629 | pubmed:author | pubmed-author:AndersonJ MJM | lld:pubmed |
| pubmed-article:2141629 | pubmed:author | pubmed-author:LeBienT WTW | lld:pubmed |
| pubmed-article:2141629 | pubmed:author | pubmed-author:MoseleyMM | lld:pubmed |
| pubmed-article:2141629 | pubmed:author | pubmed-author:LawC LCL | lld:pubmed |
| pubmed-article:2141629 | pubmed:author | pubmed-author:VillablancaJ... | lld:pubmed |
| pubmed-article:2141629 | pubmed:author | pubmed-author:ElstromR LRL | lld:pubmed |
| pubmed-article:2141629 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2141629 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:2141629 | pubmed:volume | 172 | lld:pubmed |
| pubmed-article:2141629 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2141629 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2141629 | pubmed:pagination | 325-34 | lld:pubmed |
| pubmed-article:2141629 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:2141629 | pubmed:year | 1990 | lld:pubmed |
| pubmed-article:2141629 | pubmed:articleTitle | Differentiation of normal human pre-B cells in vitro. | lld:pubmed |
| pubmed-article:2141629 | pubmed:affiliation | Department of Pediatrics, University of Minnesota Medical School, Minneapolis 55455. | lld:pubmed |
| pubmed-article:2141629 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2141629 | pubmed:publicationType | In Vitro | lld:pubmed |
| pubmed-article:2141629 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:2141629 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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