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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1990-8-1
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pubmed:abstractText |
The differentiation of surface Ig- pre-B cells into surface Ig+ B cells is a critical transition in mammalian B cell ontogeny. Elucidation of the growth factor requirements and differentiative potential of human pre-B cells has been hampered by the absence of a reproducible culture system that supports differentiation. Fluorescence-activated cell sorting and magnetic bead depletion were used to purify fetal bone marrow CD10+/surface mu- cells, which contain 60-70% cytoplasmic mu+ pre-B cells. CD10+/surface mu- cells cultured for 2 d were observed to differentiate into surface mu+ cells. Analysis by Southern blotting provided direct evidence that rearrangement of kappa light chain genes occurs in culture, and flow cytometric analysis revealed the appearance of surface Ig+ B cells expressing mu/kappa or mu/lambda. Unexpectedly, the kappa/lambda ratio in differentiated cells was the inverse of what is normally observed in adult peripheral blood. Differentiation occurs in the absence of exogenous growth factors or cytokines, suggesting that a stimulus-independent differentiative inertia might characterize pre-B cells in vivo. Future use of this model will facilitate our understanding of normal and abnormal human pre-B cell differentiation.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2141629-2465037,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2141629-2501791,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2141629-2521388,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2141629-2565353,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2141629-2642502,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2141629-2653368,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2141629-2657229,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2141629-2686678,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2141629-2848865,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2141629-2947644,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2141629-3114643,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2141629-3117132,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2141629-3162813,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2141629-3257397,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2141629-3260922,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2141629-3318292,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2141629-3871452,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2141629-3871812,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2141629-6600632,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2141629-6783958,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2141629-6783959,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2141629-6818320,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2141629-6969748,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2141629-6972409,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2141629-6972837,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2141629-6982414
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0022-1007
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
172
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
325-34
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2141629-Antigens, Differentiation,
pubmed-meshheading:2141629-Antigens, Neoplasm,
pubmed-meshheading:2141629-Antigens, Surface,
pubmed-meshheading:2141629-B-Lymphocytes,
pubmed-meshheading:2141629-Blotting, Southern,
pubmed-meshheading:2141629-Bone Marrow Cells,
pubmed-meshheading:2141629-Cell Cycle,
pubmed-meshheading:2141629-Cell Differentiation,
pubmed-meshheading:2141629-Cells, Cultured,
pubmed-meshheading:2141629-Fetus,
pubmed-meshheading:2141629-Flow Cytometry,
pubmed-meshheading:2141629-Gene Expression,
pubmed-meshheading:2141629-Gene Rearrangement,
pubmed-meshheading:2141629-Hematopoietic Stem Cells,
pubmed-meshheading:2141629-Humans,
pubmed-meshheading:2141629-Immunoglobulin kappa-Chains,
pubmed-meshheading:2141629-Neprilysin
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pubmed:year |
1990
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pubmed:articleTitle |
Differentiation of normal human pre-B cells in vitro.
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pubmed:affiliation |
Department of Pediatrics, University of Minnesota Medical School, Minneapolis 55455.
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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