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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
10
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pubmed:dateCreated |
2011-5-30
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pubmed:abstractText |
U1 Adaptors are a recently reported novel approach for targeted reduction of mRNA transcripts. A U1 adaptor oligonucleotide comprising of a target-complimentary hybridization domain and a U1 recruitment domain, directs the U1 snRNP complex to the terminal exon of a targeted gene, subsequently inhibiting poly(A) tail addition and leading to degradation of that RNA species within the nucleus. Here, we present data demonstrating U1 adapter-mediated gene silencing can result in significant 'off-target' silencing effects as demonstrated by the reduction of multiple mRNA species that were not intended to be targeted. Our data suggest that a substantial portion of this U1 adaptor-mediated off-target mRNA reduction is the result of sequestration U1 snRNP at levels sufficient to affect splicing and processing of non-target transcripts.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/21415007-10328763,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21415007-10497183,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21415007-11046128,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21415007-11682119,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21415007-11925564,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21415007-12500975,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21415007-12710670,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21415007-1400312,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21415007-15461798,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21415007-15685193,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21415007-15840817,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21415007-16385450,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21415007-16517734,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21415007-16682560,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21415007-17355180,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21415007-17576681,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21415007-17932061,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21415007-18158895,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21415007-18299285,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21415007-18463711,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21415007-19169258,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21415007-19219028,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21415007-20881964,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21415007-2529976,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21415007-3757028,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21415007-8035806,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21415007-8662854,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21415007-9115264,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21415007-9659922
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Oligonucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/Oligonucleotides, Antisense,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Small Nuclear,
http://linkedlifedata.com/resource/pubmed/chemical/RNA Precursors,
http://linkedlifedata.com/resource/pubmed/chemical/Ribonucleoprotein, U1 Small Nuclear,
http://linkedlifedata.com/resource/pubmed/chemical/U1 small nuclear RNA
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
1362-4962
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
39
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
e71
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pubmed:meshHeading |
pubmed-meshheading:21415007-Down-Regulation,
pubmed-meshheading:21415007-Gene Silencing,
pubmed-meshheading:21415007-HeLa Cells,
pubmed-meshheading:21415007-Humans,
pubmed-meshheading:21415007-Oligonucleotides,
pubmed-meshheading:21415007-Oligonucleotides, Antisense,
pubmed-meshheading:21415007-RNA, Messenger,
pubmed-meshheading:21415007-RNA, Small Nuclear,
pubmed-meshheading:21415007-RNA Precursors,
pubmed-meshheading:21415007-RNA Processing, Post-Transcriptional,
pubmed-meshheading:21415007-RNA Splicing,
pubmed-meshheading:21415007-Ribonucleoprotein, U1 Small Nuclear
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pubmed:year |
2011
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pubmed:articleTitle |
U1 adaptors result in reduction of multiple pre-mRNA species principally by sequestering U1snRNP.
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pubmed:affiliation |
Department of Core Antisense Research, ISIS Pharmaceuticals, Inc, 1896 Rutherford Road, Carlsbad, CA 92008, USA. tvickers@isisph.com
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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