Linked Life Data

21410764

Source:http://linkedlifedata.com/resource/pubmed/id/21410764

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2011-3-17
pubmed:abstractText
SOX2 is an embryonic neural crest stem-cell transcription factor recently shown to be expressed in human melanoma and to correlate with experimental tumor growth. SOX2 binds to an enhancer region of the gene that encodes for nestin, also a neural progenitor cell biomarker. To define further the potential relationship between SOX2 and nestin, we examined co-expression patterns in 135 melanomas and 37 melanocytic nevi. Immunohistochemical staining in 27 melanoma tissue sections showed an association between SOX2 positivity, spindle cell shape and a peripheral nestin distribution pattern. In contrast, SOX2-negative cells were predominantly epithelioid, and exhibited a cytoplasmic pattern for nestin. In tissue microarrays, co-expression correlated with tumor progression, with only 11% of nevi co-expressing SOX2 and nestin in contrast to 65% of metastatic melanomas, and preliminarily, with clinical outcome. Human melanoma lines that differentially expressed constitutive SOX2 revealed a positive correlation between SOX2 and nestin expression. Experimental melanomas grown from these respective cell lines in murine subcutis and dermis of xenografted human skin maintained the association between SOX2-positivity, spindle cell shape, and peripheral nestin distribution. Moreover, the cytoplasmic pattern of nestin distribution was observed in xenografts generated from SOX2-knockdown A2058 melanoma cells, in contrast to the peripheral nestin pattern seen in tumors grown from A2058 control cells transfected with non-target shRNA. In aggregate, these data further support a biologically significant linkage between SOX2 and nestin expression in human melanoma.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1600-0625
pubmed:author
pubmed:copyrightInfo
© 2011 John Wiley & Sons A/S.
pubmed:issnType
Electronic
pubmed:volume
20
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
339-45
pubmed:dateRevised
2011-6-16
pubmed:meshHeading
pubmed-meshheading:21410764-Animals, pubmed-meshheading:21410764-Chi-Square Distribution, pubmed-meshheading:21410764-Humans, pubmed-meshheading:21410764-Immunohistochemistry, pubmed-meshheading:21410764-Intermediate Filament Proteins, pubmed-meshheading:21410764-Kaplan-Meier Estimate, pubmed-meshheading:21410764-Male, pubmed-meshheading:21410764-Melanoma, pubmed-meshheading:21410764-Mice, pubmed-meshheading:21410764-Mice, SCID, pubmed-meshheading:21410764-Neoplasm Metastasis, pubmed-meshheading:21410764-Nerve Tissue Proteins, pubmed-meshheading:21410764-Nevus, Pigmented, pubmed-meshheading:21410764-SOXB1 Transcription Factors, pubmed-meshheading:21410764-Skin Neoplasms, pubmed-meshheading:21410764-Statistics, Nonparametric, pubmed-meshheading:21410764-Transplantation, Heterologous, pubmed-meshheading:21410764-Tumor Cells, Cultured
pubmed:year
2011
pubmed:articleTitle
SOX2 and nestin expression in human melanoma: an immunohistochemical and experimental study.
pubmed:affiliation
Department of Pathology, Program in Dermatopathology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.
pubmed:publicationType
Journal Article, Research Support, N.I.H., Extramural