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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1990-6-29
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pubmed:abstractText |
Complexes of murine monoclonal anti-DNP IgE and DNP-OVA interact with murine B cells to stimulate expression of cell surface Ia antigens. Enhanced membrane expression of class II MHC antigens was accompanied by a threefold increase of I-A and I-E transcripts, as measured by Northern blot. Peak accumulation of Ia mRNA were detected after 6 hr of incubation with IgE-antigen complexes and returned to control levels after 12 hr of incubation. Hence, induction of Ia mRNA by IgE-antigen complexes was compatible with cell surface Ia expression, both quantitatively and with regard to the time frame. The Ia-inductive effects of both IL-4 and IgE-antigen complexes were inhibited by cycloheximide and actinomycin-D. However, whereas actinomycin-D and cycloheximide blocked IL-4 induction of Fc epsilon RII expression, inhibition of transcription or protein synthesis did not abrogate the increased expression of Fc epsilon R associated with IgE-antigen complexes. These results suggest that the IgE-antigen-induction of B cell Ia expression follows from activation of transcription and de novo synthesis of Ia antigens.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigen-Antibody Complex,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Differentiation,
http://linkedlifedata.com/resource/pubmed/chemical/Cycloheximide,
http://linkedlifedata.com/resource/pubmed/chemical/Dactinomycin,
http://linkedlifedata.com/resource/pubmed/chemical/Histocompatibility Antigens Class II,
http://linkedlifedata.com/resource/pubmed/chemical/I-E-antigen,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin E,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-4,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Fc,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, IgG
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0008-8749
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
128
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
198-208
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:2140531-Animals,
pubmed-meshheading:2140531-Antigen-Antibody Complex,
pubmed-meshheading:2140531-Antigens, Differentiation,
pubmed-meshheading:2140531-B-Lymphocytes,
pubmed-meshheading:2140531-Cycloheximide,
pubmed-meshheading:2140531-Dactinomycin,
pubmed-meshheading:2140531-Gene Expression,
pubmed-meshheading:2140531-Histocompatibility Antigens Class II,
pubmed-meshheading:2140531-Immunoglobulin E,
pubmed-meshheading:2140531-Interleukin-4,
pubmed-meshheading:2140531-Male,
pubmed-meshheading:2140531-Mice,
pubmed-meshheading:2140531-Mice, Inbred BALB C,
pubmed-meshheading:2140531-Protein Biosynthesis,
pubmed-meshheading:2140531-RNA, Messenger,
pubmed-meshheading:2140531-Receptors, Fc,
pubmed-meshheading:2140531-Receptors, IgG,
pubmed-meshheading:2140531-Spleen
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pubmed:year |
1990
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pubmed:articleTitle |
The induction of murine B cell Ia by IgE-antigen complexes is dependent on protein synthesis and preceded by class II mRNA accumulation.
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pubmed:affiliation |
Division of Immunology, Medical Biology Institute, La Jolla, California 92037.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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