| pubmed-article:21402772 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:21402772 | lifeskim:mentions | umls-concept:C2752508 | lld:lifeskim |
| pubmed-article:21402772 | lifeskim:mentions | umls-concept:C0596402 | lld:lifeskim |
| pubmed-article:21402772 | lifeskim:mentions | umls-concept:C0001271 | lld:lifeskim |
| pubmed-article:21402772 | lifeskim:mentions | umls-concept:C1167322 | lld:lifeskim |
| pubmed-article:21402772 | lifeskim:mentions | umls-concept:C0037083 | lld:lifeskim |
| pubmed-article:21402772 | lifeskim:mentions | umls-concept:C1710082 | lld:lifeskim |
| pubmed-article:21402772 | lifeskim:mentions | umls-concept:C1314939 | lld:lifeskim |
| pubmed-article:21402772 | lifeskim:mentions | umls-concept:C0127400 | lld:lifeskim |
| pubmed-article:21402772 | pubmed:issue | 6 | lld:pubmed |
| pubmed-article:21402772 | pubmed:dateCreated | 2011-6-1 | lld:pubmed |
| pubmed-article:21402772 | pubmed:abstractText | Actin cytoskeleton has been shown to play a regulating role in several signaling pathways, and disruption of actin filament has been reported to increase sTNF-?-induced cell death. However, whether actin is involved in tmTNF-?-mediated cytotoxicity remains unclear. Here, we demonstrated that pretreatment of HL-60 with CytD or LatA to depolymerize actin significantly suppressed tmTNF-?-mediated apoptosis. Interestingly, tmTNF-? increased the actin immunoprecipitated by anti-TNFR2 but not anti-TNFR1 antibody, and disruption of the actin filament totally blocked this effect. In addition, TNFR1 knockdown by siRNA did not affect tmTNF-?-mediated cytotoxicity and the inhibitory effect of CytD, suggesting that the involvement of actin in the tmTNF-?-induced apoptosis is linked to the TNFR2 pathway. Our results revealed further that tmTNF-? signaled the inhibition of I?B degradation and NF-?B activity by recruiting RIP1 to and uncoupling TRAF2 from the TNFR2 complex. Nevertheless, CytD totally reversed the tmTNF-? signaling and activated NF-?B by recruiting TRAF2 to and dissociating RIP1 from the TNFR2 complex. Furthermore, tmTNF-? led to activation of caspase-8 by dissociation of cFLIP from TNFR2 and inhibition of the cFLIP expression. Activated caspase-8 cleft RIP1 to suppress NF-?B activity and also mediated tmTNF-?-induced apoptosis. However, CytD blocked the tmTNF-?-induced uncoupling of cFLIP from TNFR2 and prevented caspase-8 activation and the resulting cleavage of RIP1, converting the signaling for tmTNF-?-mediated apoptosis into one for activating NF-?B to survive. These results suggest that the actin cytoskeleton functions in transmitting signals via TNFR2 to mediate tmTNF-?-induced apoptosis. | lld:pubmed |
| pubmed-article:21402772 | pubmed:language | eng | lld:pubmed |
| pubmed-article:21402772 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21402772 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:21402772 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21402772 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21402772 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21402772 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21402772 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21402772 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21402772 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21402772 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21402772 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21402772 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21402772 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21402772 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21402772 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21402772 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21402772 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21402772 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21402772 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21402772 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21402772 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21402772 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21402772 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:21402772 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:21402772 | pubmed:issn | 1938-3673 | lld:pubmed |
| pubmed-article:21402772 | pubmed:author | pubmed-author:ZakTT | lld:pubmed |
| pubmed-article:21402772 | pubmed:author | pubmed-author:ZhangHailongH | lld:pubmed |
| pubmed-article:21402772 | pubmed:author | pubmed-author:FUSTBB | lld:pubmed |
| pubmed-article:21402772 | pubmed:author | pubmed-author:YangKunK | lld:pubmed |
| pubmed-article:21402772 | pubmed:author | pubmed-author:WangJingJ | lld:pubmed |
| pubmed-article:21402772 | pubmed:author | pubmed-author:LiQingfenQ | lld:pubmed |
| pubmed-article:21402772 | pubmed:author | pubmed-author:WIRTR DRD | lld:pubmed |
| pubmed-article:21402772 | pubmed:author | pubmed-author:LiuLiliL | lld:pubmed |
| pubmed-article:21402772 | pubmed:author | pubmed-author:LiuNaN | lld:pubmed |
| pubmed-article:21402772 | pubmed:author | pubmed-author:ZAKR LRL | lld:pubmed |
| pubmed-article:21402772 | pubmed:author | pubmed-author:LiZhuoyaZ | lld:pubmed |
| pubmed-article:21402772 | pubmed:author | pubmed-author:YinBingjiaoB | lld:pubmed |
| pubmed-article:21402772 | pubmed:author | pubmed-author:GaoTaoT | lld:pubmed |
| pubmed-article:21402772 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:21402772 | pubmed:volume | 89 | lld:pubmed |
| pubmed-article:21402772 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:21402772 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:21402772 | pubmed:pagination | 917-26 | lld:pubmed |
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| pubmed-article:21402772 | pubmed:meshHeading | pubmed-meshheading:21402772... | lld:pubmed |
| pubmed-article:21402772 | pubmed:year | 2011 | lld:pubmed |
| pubmed-article:21402772 | pubmed:articleTitle | The involvement of ?-actin in the signaling of transmembrane TNF-?-mediated cytotoxicity. | lld:pubmed |
| pubmed-article:21402772 | pubmed:affiliation | Department of Immunology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China. | lld:pubmed |
| pubmed-article:21402772 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:21402772 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
