Linked Life Data

2140107

Source:http://linkedlifedata.com/resource/pubmed/id/2140107

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1990-6-19
pubmed:abstractText
The processing of the primary transcript of the murine T cell receptor c beta region can result in two distinct forms of protein. The optional exon, C beta O, is appended at the 5' end of the constant region and to date has been observed at low frequencies in cDNA clones from heterogeneous populations of T lymphocyte. Because individual cell lines were not analyzed in those studies, it was not known whether small numbers of T lymphocytes use the C beta O exon exclusively or instead most T-lymphocytes use the C beta O exon but at low levels. We have determined that our CTL clone, B6.cl 4, produces both C beta O+ and C beta O- functional RNA transcripts. To determine the C beta O usage in this CTL clone, we coupled cDNA synthesis with the polymerase chain reaction using oligonucleotides corresponding to sequences at the V beta 14 region and sequences corresponding to C beta O or C beta 1. The data indicate that B6.cl 4 CTL clone is able to use either splice site but uses the C beta O exon at a frequency of approximately 2-3%.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0882-0139
pubmed:author
pubmed:issnType
Print
pubmed:volume
19
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
153-61
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
Alternative splice forms of the murine T-cell receptor beta transcript in a cytotoxic T-cell line.
pubmed:affiliation
Department of Pathology, University of Medicine & Dentistry of New Jersey, Robert Wood Johnson Medical School, Piscataway 08854.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't