Linked Life Data

21400677

Source:http://linkedlifedata.com/resource/pubmed/id/21400677

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2011-3-14
pubmed:abstractText
Clostridium difficile is a Gram-positive, spore forming, anaerobic, intestinal bacterium and is the most common cause of antibiotic-associated colitis. For many years this organism was considered genetically intractable, but in the past 10 years, multiple methods have been developed or adapted for genetic manipulation of C. difficile. This unit describes the molecular techniques used for genetic modification of this organism, including methods for gene disruption, complementation, plasmid introduction and integration, and cross-species conjugations.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
1934-8533
pubmed:author
pubmed:copyrightInfo
© 2011 by John Wiley & Sons, Inc.
pubmed:issnType
Electronic
pubmed:volume
Chapter 9
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
Unit 9A.2
pubmed:meshHeading
pubmed:year
2011
pubmed:articleTitle
Genetic manipulation of Clostridium difficile.
pubmed:affiliation
Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts, USA.
pubmed:publicationType
Journal Article, Research Support, N.I.H., Extramural