Linked Life Data

214004

Source:http://linkedlifedata.com/resource/pubmed/id/214004

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1979-1-24
pubmed:abstractText
A practical procedure for the isolation of massive numbers of GV from oocytes of Xenopus laevis at various stages of oogenesis was developed. The method is simple, rapid, and easy to perform. The isolated GV possess high activity of poly(ADP-ribose) synthase. Incubation of class-A oocytes (stages V and VI) with progesterone resulted in a stimulation of the poly(ADP-ribose) synthase activity in the GV. The stimulation of enzymatic activity occurred prior to GVBD. This stimulatory effect of progesterone on enzymatic activity was blocked by cycloheximide and actinomycin D, suggesting that induction is dependent on protein and RNA synthesis. Progesterone, however, was unable to cause disintegration of GV or to stimulate poly(ADP-ribose) synthase activity of class-B oocytes (stages III and IV). This finding suggests that the oocytes must progress to a certain stage of differentiation before progesterone can induce GVBD or stimulate poly(ADP-ribose) synthase activity.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0077-8923
pubmed:author
pubmed:issnType
Print
pubmed:day
11
pubmed:volume
286
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
398-407
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1977
pubmed:articleTitle
Stimulation of poly(adenosine diphosphate ribose) synthase activity of Xenopus germinal vesicle by progesterone.
pubmed:publicationType
Journal Article, In Vitro, Research Support, U.S. Gov't, P.H.S.