Linked Life Data

21399636

Source:http://linkedlifedata.com/resource/pubmed/id/21399636

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2011-3-31
pubmed:abstractText
We demonstrate three-dimensional (3D) super-resolution imaging of stochastically switched fluorophores distributed across whole cells. By evaluating the higher moments of the diffraction spot provided by a 4Pi detection scheme, single markers can be simultaneously localized with <10 nm precision in three dimensions in a layer of 650 nm thickness at an arbitrarily selected depth in the sample. By splitting the fluorescence light into orthogonal polarization states, our 4Pi setup also facilitates the 3D nanoscopy of multiple fluorophores. Offering a combination of multicolor recording, nanoscale resolution and extended axial depth, our method substantially advances the noninvasive 3D imaging of cells and of other transparent materials.
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1548-7105
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
8
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
353-9
pubmed:meshHeading
pubmed:year
2011
pubmed:articleTitle
Two-color nanoscopy of three-dimensional volumes by 4Pi detection of stochastically switched fluorophores.
pubmed:affiliation
Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, Göttingen, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't