Linked Life Data

21397594

Source:http://linkedlifedata.com/resource/pubmed/id/21397594

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2011-4-25
pubmed:abstractText
An understanding of the number and types of progeny produced by progenitor cells during development provides a foundation for studies of when and where cell fate determination takes place. Lineal relationships can be revealed by the identification of descendents of cells that express a recombinase, such as Cre or Flp. This method provides data concerning gene expression history, but does not provide clonal resolution among the descendents. An alternative method employs retroviral labeling, which permits the identification of clones, but does not allow for the tracking of gene expression history. Here we report a combination of these methods to circumvent each method's limitations. By employing the specificity of Cre expression, and by selecting only a subset of cells with a Cre history for retroviral infection, clones with a gene expression history can be labeled. The method utilizes a conditional allele of the avian tumor virus receptor A (TVA), which allows infection of mouse cells following Cre activity, with mammalian retroviral vectors pseudotyped with the ASLV-A envelope glycoprotein (EnvA). We quantified the efficiency and specificity of this system in vivo and in vitro. We also generated a series of retroviral vectors encoding a variety of histochemical and fluorescent reporter genes that enable the tracking of mixtures of clones, thus enabling better resolution of clonal boundaries. This method and new vectors can be used to further our understanding of the gene expression patterns of progenitor cells that make particular daughter cells, as well as provide a platform for manipulating identified subsets of developing cells.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1095-564X
pubmed:author
pubmed:copyrightInfo
Copyright © 2011 Elsevier Inc. All rights reserved.
pubmed:issnType
Electronic
pubmed:day
15
pubmed:volume
353
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
309-20
pubmed:dateRevised
2011-10-28
pubmed:meshHeading
pubmed-meshheading:21397594-Animals, pubmed-meshheading:21397594-Avian Proteins, pubmed-meshheading:21397594-Base Sequence, pubmed-meshheading:21397594-Cell Lineage, pubmed-meshheading:21397594-DNA Primers, pubmed-meshheading:21397594-Embryonic Stem Cells, pubmed-meshheading:21397594-Gene Expression Regulation, Developmental, pubmed-meshheading:21397594-Genes, Reporter, pubmed-meshheading:21397594-Genetic Vectors, pubmed-meshheading:21397594-Humans, pubmed-meshheading:21397594-Integrases, pubmed-meshheading:21397594-Mice, pubmed-meshheading:21397594-Mice, Transgenic, pubmed-meshheading:21397594-NIH 3T3 Cells, pubmed-meshheading:21397594-Receptors, Virus, pubmed-meshheading:21397594-Recombinant Proteins, pubmed-meshheading:21397594-Retina, pubmed-meshheading:21397594-Retroviridae, pubmed-meshheading:21397594-Virus Internalization
pubmed:year
2011
pubmed:articleTitle
Conditional expression of the TVA receptor allows clonal analysis of descendents from Cre-expressing progenitor cells.
pubmed:affiliation
Department of Genetics, Department of Ophthamology, Howard Hughes Medical Institute, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02115, USA.
pubmed:publicationType
Journal Article, Research Support, N.I.H., Extramural