Linked Life Data

21397022

Source:http://linkedlifedata.com/resource/pubmed/id/21397022

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3-5
pubmed:dateCreated
2011-5-31
pubmed:abstractText
The 5'-untranslated region (5'-UTR) of the estrogen receptor ? (ER?) gene plays an important role in determining its tissue-specific expression. We examined the 5'-UTRs of the mouse ER? mRNA variants in depth using the Basic Local Alignment Search Tool (BLAST), rapid amplification of 5'-cDNA ends (5'-RACE) and RT-PCR. We demonstrated the presence of multiple variants containing unique 5'-UTRs. We mapped the cDNA sequences onto the mouse genome, and found that both alternative splicing from four different leader exons (A, C, F1, and H) to exon 1, and combinations of 12 internal exons (X1, X2, X3, X4, F2/X5, X6, X7, X8, X9, X10, X11, and B) generate multiple ER? transcripts. Mouse exon B, that has homologies with human exon B and rat exon 0T, was used as an internal exon, not as a leader exon. RT-PCR analysis revealed distinct expression patterns of the variants, suggesting that the alternative promoter usage and alternative splicing are regulated in a tissue-specific manner. Our results indicate that the genomic organization of the mouse ER? gene is complicated as previously shown in the rat ER? gene. In addition, both alternative promoter usage and alternative splicing contribute to the remarkable mRNA diversity of the mouse ER? gene.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
1879-1220
pubmed:author
pubmed:copyrightInfo
Copyright © 2011 Elsevier Ltd. All rights reserved.
pubmed:issnType
Electronic
pubmed:volume
125
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
211-8
pubmed:meshHeading
pubmed:year
2011
pubmed:articleTitle
Complex organization of the 5'-untranslated region of the mouse estrogen receptor ? gene: identification of numerous mRNA transcripts with distinct 5'-ends.
pubmed:affiliation
Department of Physiology, Nippon Medical School, 1 Sendagi, Bunkyo, Tokyo 113-8602, Japan. hirotaka@nms.ac.jp
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't