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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
13
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pubmed:dateCreated |
2011-3-30
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pubmed:abstractText |
It is thought that the adaptive immune system of immature organisms follows a more deterministic program of antibody creation than is found in adults. We used high-throughput sequencing to characterize the diversifying antibody repertoire in zebrafish over five developmental time points. We found that the immune system begins in a highly stereotyped state with preferential use of a small number of V (variable) D (diverse) J (joining) gene segment combinations, but that this stereotypy decreases dramatically as the zebrafish mature, with many of the top VDJ combinations observed in 2-wk-old zebrafish virtually disappearing by 1 mo. However, we discovered that, in the primary repertoire, there are strong correlations in VDJ use that increase with zebrafish maturity, suggesting that VDJ recombination involves a level of deterministic programming that is unexpected. This stereotypy is masked by the complex diversification processes of antibody maturation; the variation and lack of correlation in full repertoires between individuals appears to be derived from randomness in clonal expansion during the affinity maturation process. These data provide a window into the mechanisms of VDJ recombination and diversity creation and allow us to better understand how the adaptive immune system achieves diversity.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/21393572-10933591,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21393572-11675347,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21393572-12920303,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21393572-15685175,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21393572-1588177,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21393572-16083957,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21393572-16261174,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21393572-16551259,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21393572-1700054,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21393572-17332892,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21393572-18423198,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21393572-1903708,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21393572-19242248,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21393572-19298667,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21393572-19423829,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21393572-2261012,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21393572-3135366,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21393572-3141924,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21393572-3975629,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21393572-6092962
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
1091-6490
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
29
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pubmed:volume |
108
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5348-53
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pubmed:dateRevised |
2011-7-28
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pubmed:meshHeading |
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pubmed:year |
2011
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pubmed:articleTitle |
Determinism and stochasticity during maturation of the zebrafish antibody repertoire.
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pubmed:affiliation |
Department of Bioengineering, Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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