Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1990-5-11
pubmed:abstractText
Medium conditioned by Chinese hamster ovary (CHO) cells transfected with the simian pre-pro-TGF beta 1 cDNA contains high levels of latent TGF beta 1. The amino-terminal region of the TGF beta 1 precursor is secreted and can be detected in the conditioned medium by immunoblotting using peptide antibodies specific for amino-terminal peptides. Chemical cross-linking of CHO-conditioned medium using bis-(sulfosuccinimidyl)-suberate (BS3) followed by immunoblot analyses indicates that latent recombinant TGF beta 1 contains both the cleaved amino-terminal glycopeptide and mature TGF beta 1 polypeptide in a noncovalent association and that this association confers latency. The data presented here do not support the involvement of a unique TGF beta binding protein(s) in latent recombinant TGF beta 1. Plasmin treatment of CHO-conditioned medium resulted in the appearance of TGF beta competing activity. In addition, immunoblot analysis of plasmin-treated CHO-conditioned medium indicates that the amino-terminal glycopeptide is partially degraded and that mature TGF beta 1 is released. Thus, activation of latent TGF beta 1 may occur by proteolytic nicking within the amino-terminal glycopeptide thereby causing a disruption of tertiary structure and noncovalent bonds, which results in the release of active, mature TGF beta 1. Acid activation of latent TGF beta, in comparison, appears to be due to dissociation of the amino-terminal glycopeptide from the mature polypeptide.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/2139036-2474534, http://linkedlifedata.com/resource/pubmed/commentcorrection/2139036-2493139, http://linkedlifedata.com/resource/pubmed/commentcorrection/2139036-2526131, http://linkedlifedata.com/resource/pubmed/commentcorrection/2139036-2891188, http://linkedlifedata.com/resource/pubmed/commentcorrection/2139036-2967299, http://linkedlifedata.com/resource/pubmed/commentcorrection/2139036-2971654, http://linkedlifedata.com/resource/pubmed/commentcorrection/2139036-3162913, http://linkedlifedata.com/resource/pubmed/commentcorrection/2139036-3163692, http://linkedlifedata.com/resource/pubmed/commentcorrection/2139036-3164441, http://linkedlifedata.com/resource/pubmed/commentcorrection/2139036-3185545, http://linkedlifedata.com/resource/pubmed/commentcorrection/2139036-3276718, http://linkedlifedata.com/resource/pubmed/commentcorrection/2139036-3458465, http://linkedlifedata.com/resource/pubmed/commentcorrection/2139036-3491081, http://linkedlifedata.com/resource/pubmed/commentcorrection/2139036-3519251, http://linkedlifedata.com/resource/pubmed/commentcorrection/2139036-3861940, http://linkedlifedata.com/resource/pubmed/commentcorrection/2139036-3866579, http://linkedlifedata.com/resource/pubmed/commentcorrection/2139036-388439, http://linkedlifedata.com/resource/pubmed/commentcorrection/2139036-5432063, http://linkedlifedata.com/resource/pubmed/commentcorrection/2139036-6088525, http://linkedlifedata.com/resource/pubmed/commentcorrection/2139036-6090474, http://linkedlifedata.com/resource/pubmed/commentcorrection/2139036-6093254, http://linkedlifedata.com/resource/pubmed/commentcorrection/2139036-6208555, http://linkedlifedata.com/resource/pubmed/commentcorrection/2139036-6303865, http://linkedlifedata.com/resource/pubmed/commentcorrection/2139036-6602130
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0021-9525
pubmed:author
pubmed:issnType
Print
pubmed:volume
110
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1361-7
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
Mechanism of activation of latent recombinant transforming growth factor beta 1 by plasmin.
pubmed:affiliation
Department of Cell Biology, Vanderbilt School of Medicine, Nashville, Tennessee 37232.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.