Source:http://linkedlifedata.com/resource/pubmed/id/21389254
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
7
|
| pubmed:dateCreated |
2011-3-22
|
| pubmed:abstractText |
Tolerance induction toward allogeneic organ grafts represents one of the major aims of transplantation medicine. Stem cells are promising candidates for promoting donor-specific tolerance. In this study, we investigated the immunomodulatory properties of murine embryonic stem cells (ESCs), obtained either by in vitro fertilization (IVF-ESCs) or by nuclear transfer (NT-ESCs), in heart transplant mouse models. IVF-ESCs did not prolong the survival of fully allogeneic cardiac transplants but significantly prolonged the survival of semiallogeneic hearts from the same ESC donor strain for >100 d in 44% of the animals. However, 28% of transplanted animals infused with IVF-ESCs experienced development of a teratoma. NT-ESCs similarly prolonged semiallogeneic heart graft survival (>100 d in 40% of the animals) but were less teratogenic. By in vitro studies, IVF-ESC and NT-ESC immunoregulation was mediated both by cell contact-dependent mechanisms and by the release of soluble factors. By adding specific inhibitors, we identified PGE(2) as a soluble mediator of ESC immunoregulation. Expansion of regulatory T cells was found in lymphoid organs and in the grafts of IVF-ESC- and NT-ESC-tolerized mice. Our study demonstrates that both IVF-ESCs and NT-ESCs modulate recipient immune response toward tolerance to solid organ transplantation, and that NT-ESCs exhibit a lower tendency for teratoma formation. Because NT-ESCs are obtained by NT of a somatic cell from living individuals into an enucleated oocyte, they could represent a source of donor-derived stem cells to induce tolerance to solid organ allograft.
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| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Apr
|
| pubmed:issn |
1550-6606
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| pubmed:author |
pubmed-author:AzzolliniNadiaN,
pubmed-author:CasiraghiFedericaF,
pubmed-author:CassisPaolaP,
pubmed-author:CuginiDanielaD,
pubmed-author:GaragnaSilviaS,
pubmed-author:ImbertiBarbaraB,
pubmed-author:MorigiMarinaM,
pubmed-author:NorisMarinaM,
pubmed-author:RediCarlo AlbertoCA,
pubmed-author:RemuzziGiuseppeG,
pubmed-author:SebastianoVittorioV,
pubmed-author:SoliniSamanthaS,
pubmed-author:TodeschiniMartaM,
pubmed-author:ZuccottiMaurizioM
|
| pubmed:issnType |
Electronic
|
| pubmed:day |
1
|
| pubmed:volume |
186
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
4164-74
|
| pubmed:meshHeading |
pubmed-meshheading:21389254-Adoptive Transfer,
pubmed-meshheading:21389254-Animals,
pubmed-meshheading:21389254-Cell Line,
pubmed-meshheading:21389254-Embryonic Stem Cells,
pubmed-meshheading:21389254-Female,
pubmed-meshheading:21389254-Fertilization in Vitro,
pubmed-meshheading:21389254-Graft Rejection,
pubmed-meshheading:21389254-Graft Survival,
pubmed-meshheading:21389254-Heart Transplantation,
pubmed-meshheading:21389254-Male,
pubmed-meshheading:21389254-Mice,
pubmed-meshheading:21389254-Mice, Inbred BALB C,
pubmed-meshheading:21389254-Mice, Inbred C3H,
pubmed-meshheading:21389254-Mice, Inbred C57BL,
pubmed-meshheading:21389254-Mice, Inbred DBA,
pubmed-meshheading:21389254-Mice, Transgenic,
pubmed-meshheading:21389254-Nuclear Proteins,
pubmed-meshheading:21389254-Random Allocation,
pubmed-meshheading:21389254-Transplantation, Homologous
|
| pubmed:year |
2011
|
| pubmed:articleTitle |
Embryonic stem cells, derived either after in vitro fertilization or nuclear transfer, prolong survival of semiallogeneic heart transplants.
|
| pubmed:affiliation |
Department of Molecular Medicine, Mario Negri Institute for Pharmacological Research, Bergamo 24125, Italy.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|