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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
7
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pubmed:dateCreated |
1990-5-4
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pubmed:abstractText |
We have reexamined the balance between cell birth, cell maturation, and cell death in the thymus by labeling dividing thymocytes and their progeny in vivo with [3H]-thymidine, isolating clearly defined subpopulations by fluorescence-activated cell sorting, and determining the distribution of label by autoradiography. When mature thymocytes were precisely defined (as CD4+CD8- CD3+ or CD4-CD8+ CD3+) and separated from immature single positives (CD4+CD8- CD3- and CD4-CD8+ CD3-), a lag was observed in the rate of entry of [3H]thymidine into mature cells. Thus, many of the mature thymocytes appear to derive from a small nondividing cortical thymocyte pool, rather than originating directly from the earliest dividing CD4+CD8+ blasts. There was little evidence for cell division during or after mature thymocyte formation, suggesting a one-for-one differentiation from cortical cells rather than selective clonal expansion. The rate of production of mature single positive thymocytes agreed closely with estimates of the rate of export of mature T cells from the thymus and was only 3% of the rate of production of double-positive cortical thymocytes. This was compatible with a stringent selection process and extensive intrathymic cell death and suggested that no extensive negative selection occurred after the mature cells were formed.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2138780-2435787,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2138780-2460926,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2138780-2508090,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2138780-2547249,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2138780-2784545,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2138780-2883579,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2138780-2965990,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2138780-2966738,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2138780-2970592,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2138780-2970593,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2138780-300654,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2138780-3093565,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2138780-3126396,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2138780-3126397,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2138780-317932,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2138780-3251632,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2138780-3260350,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2138780-3261392,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2138780-3261843,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2138780-3262831,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2138780-3264054,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2138780-3494522,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2138780-4549190,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2138780-4766938,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2138780-6157714,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2138780-6442266,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2138780-7379836
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD3,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD4,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD8,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Differentiation...,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Antigen, T-Cell,
http://linkedlifedata.com/resource/pubmed/chemical/Thymidine,
http://linkedlifedata.com/resource/pubmed/chemical/Tritium
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
87
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2579-82
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2138780-Animals,
pubmed-meshheading:2138780-Antigens, CD3,
pubmed-meshheading:2138780-Antigens, CD4,
pubmed-meshheading:2138780-Antigens, CD8,
pubmed-meshheading:2138780-Antigens, Differentiation, T-Lymphocyte,
pubmed-meshheading:2138780-Cell Division,
pubmed-meshheading:2138780-DNA Replication,
pubmed-meshheading:2138780-Kinetics,
pubmed-meshheading:2138780-Male,
pubmed-meshheading:2138780-Mice,
pubmed-meshheading:2138780-Mice, Inbred CBA,
pubmed-meshheading:2138780-Receptors, Antigen, T-Cell,
pubmed-meshheading:2138780-T-Lymphocytes,
pubmed-meshheading:2138780-Thymidine,
pubmed-meshheading:2138780-Thymus Gland,
pubmed-meshheading:2138780-Tritium
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pubmed:year |
1990
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pubmed:articleTitle |
Kinetics of mature T-cell development in the thymus.
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pubmed:affiliation |
Walter and Eliza Hall Institute of Medical Research, Melbourne, Victoria, Australia.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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