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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
1990-5-4
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pubmed:abstractText |
We investigated cell cycle-dependent regulation of protein kinase activity encoded by the viral mos gene by using a normal rat kidney cell line (NRK-6m2) chronically infected with a temperature-sensitive mutant (ts110) of Moloney murine sarcoma virus, which produces the P85gag-mos transforming protein. In elutriation experiments, in which cells in various phases of the cell cycle are separated based upon size, a twofold increase in the specific activity of the P85gag-mos protein kinase was observed as cells progressed from G0/G1 through S and G2/M. A three- to fourfold increase in gas-mos protein kinase specific activity relative to unsynchronized cells was observed in mitotic NRK-6m2 cells synchronized by treatment with thymidine followed by colcemid or with nocodazole alone. Interestingly, the gag-mos protein was structurally altered in mitotic cells generating a protein species moving slower than P85gag-mos in SDS-polyacrylamide gels. Our findings indicate that viral mos protein kinase activity is regulated during the cell cycle via phosphorylation. We propose that the mos transforming protein functions in a pleiotropic manner, affecting both cytoplasmic and nuclear targets.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Amino Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Oncogene Proteins v-mos,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Tyrosine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Retroviridae Proteins, Oncogenic
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0950-9232
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
5
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
171-8
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:2138725-Amino Acids,
pubmed-meshheading:2138725-Animals,
pubmed-meshheading:2138725-Cell Cycle,
pubmed-meshheading:2138725-Cell Separation,
pubmed-meshheading:2138725-Immunoblotting,
pubmed-meshheading:2138725-Mitosis,
pubmed-meshheading:2138725-Oncogene Proteins v-mos,
pubmed-meshheading:2138725-Peptide Mapping,
pubmed-meshheading:2138725-Phosphorylation,
pubmed-meshheading:2138725-Protein-Tyrosine Kinases,
pubmed-meshheading:2138725-Rats,
pubmed-meshheading:2138725-Retroviridae Proteins, Oncogenic
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pubmed:year |
1990
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pubmed:articleTitle |
Cell cycle-mediated structural and functional alteration of P85gag-mos protein kinase activity.
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pubmed:affiliation |
Department of Molecular Pathology, University of Texas M.D. Anderson Cancer Center, Houston 77030.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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