Source:http://linkedlifedata.com/resource/pubmed/id/21384920
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
8
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pubmed:dateCreated |
2011-4-20
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pubmed:abstractText |
Moderately thermophilic bacterial strain CC-HSB-11(T) (Muricauda lutaonensis), which was described recently from a coastal hot spring of Green Island, Taiwan, has been identified to produce zeaxanthin as a predominant xanthophyll by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Cell culture in bioreactor produced 3.12 ± 0.18 mg zeaxanthin L(-1) of culture. Micronization of zeaxanthin was achieved through supercritical carbon dioxide antisolvent precipitation method. Yield of zeaxanthin after the process was 53.4%. Dynamic light scattering assay determined the polydisperse existence of micronized particles of size 3 nm to 2 ?m. Field emission scanning electron microscopy revealed distinct morphology and size distribution heterogeneity of particles. Integrity of zeaxanthin after the antisolvent process was assessed by LC-MS/MS. The technique capitalizes on the inherent ability of CC-HSB-11(T) to synthesize zeaxanthin and the work demonstrated feasibility of antisolvent precipitation method to produce microparticles exploiting a bacterial strain.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
1520-5118
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pubmed:author | |
pubmed:issnType |
Electronic
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pubmed:day |
27
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pubmed:volume |
59
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4119-24
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pubmed:meshHeading |
pubmed-meshheading:21384920-Carbon Dioxide,
pubmed-meshheading:21384920-Chromatography, High Pressure Liquid,
pubmed-meshheading:21384920-Fermentation,
pubmed-meshheading:21384920-Flavobacteriaceae,
pubmed-meshheading:21384920-Mass Spectrometry,
pubmed-meshheading:21384920-Microscopy, Electron, Scanning,
pubmed-meshheading:21384920-Spectrophotometry, Ultraviolet,
pubmed-meshheading:21384920-Xanthophylls
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pubmed:year |
2011
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pubmed:articleTitle |
Supercritical carbon dioxide micronization of zeaxanthin from moderately thermophilic bacteria Muricauda lutaonensis CC-HSB-11T.
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pubmed:affiliation |
Department of Soil & Environmental Sciences, College of Agriculture and Natural Resources, National Chung Hsing University, Taichung, Taiwan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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