Linked Life Data

21383147

Source:http://linkedlifedata.com/resource/pubmed/id/21383147

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
2011-3-23
pubmed:databankReference
pubmed:abstractText
The circadian clock is phase-delayed or -advanced by light when given at early or late subjective night, respectively. Despite the importance of the time-of-day-dependent phase responses to light, the underlying molecular mechanism is poorly understood. Here, we performed a comprehensive analysis of light-inducible genes in the chicken pineal gland, which consists of light-sensitive clock cells representing a prototype of the clock system. Light stimulated expression of 62 genes and 40 ESTs by >2.5-fold, among which genes responsive to the heat shock and endoplasmic reticulum stress as well as their regulatory transcription factors heat shock factor (HSF)1, HSF2, and X-box-binding protein 1 (XBP1) were strongly activated when a light pulse was given at late subjective night. In contrast, the light pulse at early subjective night caused prominent induction of E4bp4, a key regulator in the phase-delaying mechanism of the pineal clock, along with activation of a large group of cholesterol biosynthetic genes that are targets of sterol regulatory element-binding protein (SREBP) transcription factor. We found that the light pulse stimulated proteolytic formation of active SREBP-1 that, in turn, transactivated E4bp4 expression, linking SREBP with the light-input pathway of the pineal clock. As an output of light activation of cholesterol biosynthetic genes, we found light-stimulated pineal production of a neurosteroid, 7?-hydroxypregnenolone, demonstrating a unique endocrine function of the pineal gland. Intracerebroventricular injection of 7?-hydroxypregnenolone activated locomotor activities of chicks. Our study on the genome-wide gene expression analysis revealed time-of-day-dependent light activation of signaling pathways and provided molecular connection between gene expression and behavior through neurosteroid release from the pineal gland.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
1091-6490
pubmed:author
pubmed:issnType
Electronic
pubmed:day
22
pubmed:volume
108
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4864-9
pubmed:dateRevised
2011-9-23
pubmed:meshHeading
pubmed-meshheading:21383147-17-alpha-Hydroxypregnenolone, pubmed-meshheading:21383147-Animals, pubmed-meshheading:21383147-Base Sequence, pubmed-meshheading:21383147-Behavior, Animal, pubmed-meshheading:21383147-Chickens, pubmed-meshheading:21383147-Cholesterol, pubmed-meshheading:21383147-Circadian Rhythm, pubmed-meshheading:21383147-DNA-Binding Proteins, pubmed-meshheading:21383147-Endoplasmic Reticulum, pubmed-meshheading:21383147-Gene Expression Regulation, pubmed-meshheading:21383147-Genome-Wide Association Study, pubmed-meshheading:21383147-Heat-Shock Proteins, pubmed-meshheading:21383147-Light, pubmed-meshheading:21383147-Locomotion, pubmed-meshheading:21383147-Male, pubmed-meshheading:21383147-Molecular Sequence Data, pubmed-meshheading:21383147-Signal Transduction, pubmed-meshheading:21383147-Sterol Regulatory Element Binding Protein 1, pubmed-meshheading:21383147-Transcription Factors
pubmed:year
2011
pubmed:articleTitle
Light-dependent and circadian clock-regulated activation of sterol regulatory element-binding protein, X-box-binding protein 1, and heat shock factor pathways.
pubmed:affiliation
Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Tokyo 113-0033, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't