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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
1990-4-6
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pubmed:abstractText |
The adenovirus E4 gene encodes a trans-activating function that can stimulate the E2 promoter. E2 promoter sequences required for E4 trans-activation are identical to those required for E1A trans-activation, and these principally are the E2 promoter binding factor (E2F) binding sites. Furthermore, full activation of E2F DNA binding activity requires both E1A and E4 action. Analysis of a series of mutant E4 viruses identifies open reading frame (orf) 6/7 of the E4 transcription unit as that required for activation of E2F binding activity. In addition, the assay of various E4 cDNAs demonstrates that the E4 orf 6/7 also is responsible for the trans-activation of E2 transcription. Translation of the E4 orf 6/7 mRNA, but not a control mRNA, in a reticulocyte extract generates an activity that can stimulate cooperative binding of E2F in vitro, consistent with recent in vivo assays that demonstrate a role for the E4 gene in E2F stable complex formation. This stimulation is due to a direct interaction of the E4 protein with E2F since an antibody that recognizes the E4 orf 6/7 polypeptide detects this E4 protein in the E2F-DNA complex. We conclude that the E4 orf 6/7 product interacts with the E2F factor altering binding to allow formation of a stable complex that results in a stimulation of transcription.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2137929-2522934,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2137929-2523514,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2137929-2524598,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2137929-2527310,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2137929-2532611,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2137929-2550320,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2137929-2667136,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2137929-2726450,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2137929-2820719,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2137929-2838381,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2137929-2938741,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2137929-2942943,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2137929-2949089,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2137929-2950379,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2137929-2951737,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2137929-2963331,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2137929-2963951,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2137929-3530501,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2137929-3855557,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2137929-3857577,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2137929-4006920,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2137929-4029123,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2137929-4032537,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2137929-6088804,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2137929-6246373,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2137929-6310575,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2137929-6960240,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2137929-7166756
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
87
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2008-12
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2137929-Adenovirus Early Proteins,
pubmed-meshheading:2137929-Adenoviruses, Human,
pubmed-meshheading:2137929-Animals,
pubmed-meshheading:2137929-DNA-Binding Proteins,
pubmed-meshheading:2137929-Genes, Viral,
pubmed-meshheading:2137929-HeLa Cells,
pubmed-meshheading:2137929-Humans,
pubmed-meshheading:2137929-Oncogene Proteins, Viral,
pubmed-meshheading:2137929-Plasmids,
pubmed-meshheading:2137929-Promoter Regions, Genetic,
pubmed-meshheading:2137929-Trans-Activators,
pubmed-meshheading:2137929-Transcription, Genetic,
pubmed-meshheading:2137929-Transcriptional Activation,
pubmed-meshheading:2137929-Vero Cells,
pubmed-meshheading:2137929-Viral Structural Proteins
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pubmed:year |
1990
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pubmed:articleTitle |
An adenovirus E4 gene product trans-activates E2 transcription and stimulates stable E2F binding through a direct association with E2F.
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pubmed:affiliation |
Howard Hughes Medical Institute, Duke University Medical Center, Durham, NC 27710.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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