Linked Life Data

21378599

Source:http://linkedlifedata.com/resource/pubmed/id/21378599

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2011-5-9
pubmed:abstractText
The novel Cav1.3 (?1D) L-type Ca²? channel plays a significant role in sinoatrial (SA) and atrioventricular (AV) nodes function and in atrial fibrillation. However, the characterization of ?1D Ca²? channel during heart development is very limited. We used real-time RT-PCR, Western blotting, and indirect immunostaining to characterize the developmental expression and localization of ?1D Ca²? channel in rat hearts. Both protein and mRNA levels of ?1D Ca²? channel decreased postnatally. Two forms of ?1D Ca²? channel protein (250 and 190 kD) were observed, with the full-length (250 kD) channel protein being predominant in the prenatal stages. Both Western blots and confocal imaging demonstrated that ?1D Ca²? channel protein was expressed in both atria and ventricles at fetal and neonatal stages but was absent in the adult ventricles. Interestingly, ?1D Ca²? channel was also found at the nucleus/perinucleus of immature but not adult atrial cells. Furthermore, the nuclear staining was reproduced in adult atrial cell line, HL-1 cells, which possess immature properties. The data are first to show that ?1D Ca²? channel has unique age-dependent expression profile and subcellular localization in the heart, suggesting a developmental stage-dependent specific function.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1530-0447
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
69
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
479-84
pubmed:meshHeading
pubmed:year
2011
pubmed:articleTitle
Perinatal and postnatal expression of Cav1.3 ?1D Ca²? channel in the rat heart.
pubmed:affiliation
Molecular and Cellular Cardiology Program, VA New York Harbor Healthcare System, Brooklyn, NY 11209, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, N.I.H., Extramural