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rdf:type |
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lifeskim:mentions |
umls-concept:C0018850,
umls-concept:C0026926,
umls-concept:C0039194,
umls-concept:C0086418,
umls-concept:C0229664,
umls-concept:C0439097,
umls-concept:C1264633,
umls-concept:C1547348,
umls-concept:C1552644,
umls-concept:C1705241,
umls-concept:C1823153,
umls-concept:C1879547,
umls-concept:C2349976
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pubmed:issue |
3
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pubmed:dateCreated |
1990-4-12
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pubmed:abstractText |
We report that M. tuberculosis organisms, but neither PHA nor allogeneic stimulator cells, preferentially activate gamma/delta+ cells within E rosette-purified peripheral blood T cells. gamma/delta+ T cells from purified protein derivative (PPD)-nonimmune healthy donors were enriched by depletion of CD4+ and CD8+ cells; double-negative (DN) cells contained 65-92% gamma/delta+ T cells. Limiting dilution (LD) analyses revealed that 1 of 2-19 purified DN cells proliferated in response to mycobacteria, while frequencies of DN cells proliferating in response to a recombinant 65-kD heat shock protein (hsp 65) of M. tuberculosis/M. bovis were 10-20-fold lower. Established clones of mycobacteria-reactive gamma/delta+ T cells specifically recognized mycobacteria, but neither PPD nor hsp 65. Restimulation of these clones required the presence of PBMC feeder cells; EBV-transformed lymphoblastoid cell lines could not substitute for PBMC. Mycobacteria-reactive gamma/delta+ clones proliferated equally well in the presence of autologous or allogeneic (HLA-DR-different) PBMC feeder cells and thus were not MHC class II restricted. Taken together, these results demonstrate that mycobacteria-reactive gamma/delta+ T cells are present in high frequency in the peripheral blood of healthy individuals, and suggest that hsp 65 of mycobacteria is not a major antigen for gamma/delta+ T cells of normal PPD-nonimmune blood donors.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2137854-2413529,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2137854-2433335,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2137854-2434855,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2137854-2440673,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2137854-2443600,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2137854-2450164,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2137854-2462503,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2137854-2466078,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2137854-2469770,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2137854-2471938,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2137854-2473405,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2137854-2521300,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2137854-2522086,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2137854-2524009,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2137854-2524098,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2137854-2524273,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2137854-2525421,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2137854-2564416,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2137854-2568584,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2137854-2569027,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2137854-2646373,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2137854-2653369,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2137854-2785131,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2137854-2788923,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2137854-2803504,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2137854-2846743,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2137854-2885839,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2137854-2892795,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2137854-2965741,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2137854-2968521,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2137854-2978457,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2137854-3043226,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2137854-3102966,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2137854-3102967,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2137854-3106059,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2137854-3499575,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2137854-3512760,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2137854-351618,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2137854-3672118,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2137854-3905615,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2137854-7009746
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0022-1007
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
171
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
667-79
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2137854-HLA-DR Antigens,
pubmed-meshheading:2137854-Heat-Shock Proteins,
pubmed-meshheading:2137854-Humans,
pubmed-meshheading:2137854-Lymphocyte Activation,
pubmed-meshheading:2137854-Mycobacterium tuberculosis,
pubmed-meshheading:2137854-Receptors, Antigen, T-Cell,
pubmed-meshheading:2137854-Receptors, Antigen, T-Cell, gamma-delta,
pubmed-meshheading:2137854-T-Lymphocytes,
pubmed-meshheading:2137854-Tuberculin
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pubmed:year |
1990
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pubmed:articleTitle |
A large fraction of human peripheral blood gamma/delta + T cells is activated by Mycobacterium tuberculosis but not by its 65-kD heat shock protein.
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pubmed:affiliation |
Institute of Immunology, University of Heidelberg, Federal Republic of Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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