Source:http://linkedlifedata.com/resource/pubmed/id/21378272
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
17
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| pubmed:dateCreated |
2011-4-29
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| pubmed:abstractText |
A transient erythromyeloid wave of definitive hematopoietic progenitors (erythroid/myeloid progenitors [EMPs]) emerges in the yolk sac beginning at embryonic day 8.25 (E8.25) and colonizes the liver by E10.5, before adult-repopulating hematopoietic stem cells. At E11.5, we observe all maturational stages of erythroid precursors in the liver and the first definitive erythrocytes in the circulation. These early fetal liver erythroblasts express predominantly adult ?-globins and the definitive erythroid-specific transcriptional modifiers c-myb, Sox6, and Bcl11A. Surprisingly, they also express low levels of "embryonic" ?H1-, but not ?y-, globin transcripts. Consistent with these results, RNA polymerase and highly modified histones are found associated with ?H1- and adult globin, but not ?y-globin, genes. E11.5 definitive proerythroblasts from mice transgenic for the human ?-globin locus, like human fetal erythroblasts, express predominately human ?-, low ?-, and no ?-globin transcripts. Significantly, E9.5 yolk sac-derived EMPs cultured in vitro have similar murine and human transgenic globin expression patterns. Later liver proerythroblasts express low levels of ?-globin, while adult marrow proerythroblasts express only ?-globin transcripts. We conclude that yolk sac-derived EMPs, the first of 2 origins of definitive erythropoiesis, express a unique pattern of globin genes as they generate the first definitive erythrocytes in the liver of the mammalian embryo.
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| pubmed:grant | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
AIM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/GATA1 Transcription Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Gata1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Kruppel-Like Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/beta-Globins,
http://linkedlifedata.com/resource/pubmed/chemical/erythroid Kruppel-like factor
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| pubmed:status |
MEDLINE
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| pubmed:month |
Apr
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| pubmed:issn |
1528-0020
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| pubmed:author | |
| pubmed:issnType |
Electronic
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| pubmed:day |
28
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| pubmed:volume |
117
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
4600-8
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| pubmed:dateRevised |
2011-8-5
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| pubmed:meshHeading |
pubmed-meshheading:21378272-Animals,
pubmed-meshheading:21378272-Animals, Outbred Strains,
pubmed-meshheading:21378272-Cell Lineage,
pubmed-meshheading:21378272-Erythroblasts,
pubmed-meshheading:21378272-Erythrocytes,
pubmed-meshheading:21378272-Erythroid Cells,
pubmed-meshheading:21378272-Erythropoiesis,
pubmed-meshheading:21378272-GATA1 Transcription Factor,
pubmed-meshheading:21378272-Gene Expression Regulation, Developmental,
pubmed-meshheading:21378272-Hematopoietic Stem Cells,
pubmed-meshheading:21378272-Humans,
pubmed-meshheading:21378272-Kruppel-Like Transcription Factors,
pubmed-meshheading:21378272-Liver,
pubmed-meshheading:21378272-Mammals,
pubmed-meshheading:21378272-Mice,
pubmed-meshheading:21378272-Mice, Transgenic,
pubmed-meshheading:21378272-Yolk Sac,
pubmed-meshheading:21378272-beta-Globins
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| pubmed:year |
2011
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| pubmed:articleTitle |
A transient definitive erythroid lineage with unique regulation of the ?-globin locus in the mammalian embryo.
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| pubmed:affiliation |
Department of Pediatrics, Center for Pediatric Biomedical Research, University of Rochester Medical Center, Rochester, NY, USA.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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