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rdf:type |
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lifeskim:mentions |
umls-concept:C0017362,
umls-concept:C0027923,
umls-concept:C0028158,
umls-concept:C0033684,
umls-concept:C0080347,
umls-concept:C0205164,
umls-concept:C1514562,
umls-concept:C1880389,
umls-concept:C1883204,
umls-concept:C1883221,
umls-concept:C2700640
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pubmed:issue |
3
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pubmed:dateCreated |
1990-3-26
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pubmed:databankReference |
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pubmed:abstractText |
The nitrogen regulatory circuit of Neurospora crassa consists of a set of unlinked structural genes which specify various nitrogen catabolic enzymes plus control genes and metabolic effectors which regulate their expression. The positive-acting nit-2 regulatory gene is required to turn on the expression of the nitrogen catabolic enzymes during conditions of nitrogen limitation. The complete nucleotide sequence of the nit-2 gene was determined. The nit-2 mRNA is 4.3 kilobases long and has a long nontranslated sequence at both its 5' and 3' ends. The nit-2 gene nucleotide sequence can be translated to yield a protein containing 1,036 amino acid residues with a molecular weight of approximately 110,000. Deletion analyses demonstrated that approximately 21% of the NIT2 protein at its carboxy terminus can be removed without loss of function. The nit-2 protein contains a single putative Cys2/Cys2 zinc finger domain which appears to function in DNA binding and which has striking homology to a mammalian trans-acting factor, GF-1.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2137552-124058,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2137552-2567729,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2137552-271968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2137552-2725678,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2137552-2879771,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2137552-2885741,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2137552-2891138,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2137552-2906403,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2137552-2952636,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2137552-2954160,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2137552-2962990,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2137552-2965995,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2137552-2967496,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2137552-3028647,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2137552-3146691,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2137552-3299266,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2137552-3530496,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2137552-3709534,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2137552-3881765,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2137552-388356,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2137552-4504350,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2137552-6107286,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2137552-6109228,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2137552-6117784,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2137552-6238946,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2137552-6366516,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2137552-6433345,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2137552-6450203,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2137552-6457234,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2137552-881736,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2137552-922889,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2137552-9279395
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0270-7306
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
10
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1056-65
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2137552-Amino Acid Sequence,
pubmed-meshheading:2137552-Base Sequence,
pubmed-meshheading:2137552-DNA Mutational Analysis,
pubmed-meshheading:2137552-DNA-Binding Proteins,
pubmed-meshheading:2137552-Genes, Fungal,
pubmed-meshheading:2137552-Metalloproteins,
pubmed-meshheading:2137552-Molecular Sequence Data,
pubmed-meshheading:2137552-Neurospora,
pubmed-meshheading:2137552-Neurospora crassa,
pubmed-meshheading:2137552-Nitrogen Fixation,
pubmed-meshheading:2137552-Restriction Mapping
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pubmed:year |
1990
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pubmed:articleTitle |
nit-2, the major nitrogen regulatory gene of Neurospora crassa, encodes a protein with a putative zinc finger DNA-binding domain.
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pubmed:affiliation |
Department of Biochemistry, Ohio State University, Columbus 43210-1292.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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