| pubmed-article:21371768 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:21371768 | lifeskim:mentions | umls-concept:C0018150 | lld:lifeskim |
| pubmed-article:21371768 | lifeskim:mentions | umls-concept:C0205148 | lld:lifeskim |
| pubmed-article:21371768 | lifeskim:mentions | umls-concept:C0443254 | lld:lifeskim |
| pubmed-article:21371768 | lifeskim:mentions | umls-concept:C0449851 | lld:lifeskim |
| pubmed-article:21371768 | lifeskim:mentions | umls-concept:C1883709 | lld:lifeskim |
| pubmed-article:21371768 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:21371768 | pubmed:dateCreated | 2011-3-29 | lld:pubmed |
| pubmed-article:21371768 | pubmed:abstractText | Colony counting by spreading bacterial suspensions on plating media by various techniques is of general concern. Comparative studies between hand plating (Drigalski-spatula technique) for different time intervals and spiral plating resulted in significant differences in colony counts. Lower counts of Gram-negative bacteria were obtained by using hand plating for more than 10s, compared with short time hand plating (5s) or spiral plating. Colony counting of Gram-positive bacteria showed no differences between both techniques. Further characterisation of Escherichia coli cells spread with the Drigalski-spatula technique by electron microscopy revealed a large number of damaged cells compared to control samples. The data clearly shows that the mechanical forces during hand plating are sufficient to damage E. coli cells. | lld:pubmed |
| pubmed-article:21371768 | pubmed:language | eng | lld:pubmed |
| pubmed-article:21371768 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21371768 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:21371768 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:21371768 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:21371768 | pubmed:issn | 1879-3460 | lld:pubmed |
| pubmed-article:21371768 | pubmed:author | pubmed-author:MüllerRolfR | lld:pubmed |
| pubmed-article:21371768 | pubmed:author | pubmed-author:HedderichRein... | lld:pubmed |
| pubmed-article:21371768 | pubmed:author | pubmed-author:ReissbrodtRol... | lld:pubmed |
| pubmed-article:21371768 | pubmed:author | pubmed-author:BannertNorber... | lld:pubmed |
| pubmed-article:21371768 | pubmed:author | pubmed-author:HollandGudrun... | lld:pubmed |
| pubmed-article:21371768 | pubmed:author | pubmed-author:KaiserPetraP | lld:pubmed |
| pubmed-article:21371768 | pubmed:author | pubmed-author:GreulichYasmi... | lld:pubmed |
| pubmed-article:21371768 | pubmed:copyrightInfo | Copyright © 2011 Elsevier B.V. All rights reserved. | lld:pubmed |
| pubmed-article:21371768 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:21371768 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:21371768 | pubmed:volume | 146 | lld:pubmed |
| pubmed-article:21371768 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:21371768 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:21371768 | pubmed:pagination | 105-7 | lld:pubmed |
| pubmed-article:21371768 | pubmed:meshHeading | pubmed-meshheading:21371768... | lld:pubmed |
| pubmed-article:21371768 | pubmed:meshHeading | pubmed-meshheading:21371768... | lld:pubmed |
| pubmed-article:21371768 | pubmed:meshHeading | pubmed-meshheading:21371768... | lld:pubmed |
| pubmed-article:21371768 | pubmed:meshHeading | pubmed-meshheading:21371768... | lld:pubmed |
| pubmed-article:21371768 | pubmed:year | 2011 | lld:pubmed |
| pubmed-article:21371768 | pubmed:articleTitle | Mechanical damage to Gram-negative bacteria by surface plating with the Drigalski-spatula technique. | lld:pubmed |
| pubmed-article:21371768 | pubmed:affiliation | heipha Dr. Müller GmbH, Eppelheim, Germany. reiner.hedderich@heipha.de | lld:pubmed |
| pubmed-article:21371768 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:21371768 | pubmed:publicationType | Comparative Study | lld:pubmed |
