Linked Life Data

21371768

Source:http://linkedlifedata.com/resource/pubmed/id/21371768

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2011-3-29
pubmed:abstractText
Colony counting by spreading bacterial suspensions on plating media by various techniques is of general concern. Comparative studies between hand plating (Drigalski-spatula technique) for different time intervals and spiral plating resulted in significant differences in colony counts. Lower counts of Gram-negative bacteria were obtained by using hand plating for more than 10s, compared with short time hand plating (5s) or spiral plating. Colony counting of Gram-positive bacteria showed no differences between both techniques. Further characterisation of Escherichia coli cells spread with the Drigalski-spatula technique by electron microscopy revealed a large number of damaged cells compared to control samples. The data clearly shows that the mechanical forces during hand plating are sufficient to damage E. coli cells.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
1879-3460
pubmed:author
pubmed:copyrightInfo
Copyright © 2011 Elsevier B.V. All rights reserved.
pubmed:issnType
Electronic
pubmed:day
15
pubmed:volume
146
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
105-7
pubmed:meshHeading
pubmed:year
2011
pubmed:articleTitle
Mechanical damage to Gram-negative bacteria by surface plating with the Drigalski-spatula technique.
pubmed:affiliation
heipha Dr. Müller GmbH, Eppelheim, Germany. reiner.hedderich@heipha.de
pubmed:publicationType
Journal Article, Comparative Study