2137089

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1

Several investigations have demonstrated the ability of synthetic peptides homologous to the nuclear transport signal of simian virus 40 large T antigen to induce the nuclear transport of nonnuclear carrier proteins. To determine the generality of peptide-induced transport, six peptides with sequences derived from four previously identified nuclear transport signals were synthesized and examined for their ability to induce the transport of mouse immunoglobulin G following microinjection into the cytoplasm of mammalian cells. Peptides containing transport signals from simian virus 40 T antigen, Xenopus nucleoplasmin, and adenovirus E1A proteins were highly efficient at peptide-induced transport, while a peptide homologous to yeast MAT alpha 2 protein was incapable of inducing transport. A short nucleoplasmin peptide that contained only the basic amino acid domain was capable of inducing transport but yielded a much slower rate of transport than a long nucleoplasmin peptide encompassing the previously identified minimal transport signal. The short nucleoplasmin signal exhibited a greater capacity for transport than a peptide homologous to the cytoplasmic mutant T antigen signal when conjugates with a low number of signals coupled per carrier protein were examined. However, the short nucleoplasmin peptide was only marginally more effective than the T antigen mutant peptide when conjugates with a high number of signals coupled per carrier protein were examined.

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0014-4827

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32-8

pubmed-meshheading:2137089-Adenovirus Early Proteins, pubmed-meshheading:2137089-Amino Acid Sequence, pubmed-meshheading:2137089-Antigens, Polyomavirus Transforming, pubmed-meshheading:2137089-Biological Transport, pubmed-meshheading:2137089-Carrier Proteins, pubmed-meshheading:2137089-Cell Nucleus, pubmed-meshheading:2137089-Fungal Proteins, pubmed-meshheading:2137089-Homeodomain Proteins, pubmed-meshheading:2137089-Microinjections, pubmed-meshheading:2137089-Molecular Sequence Data, pubmed-meshheading:2137089-Nuclear Proteins, pubmed-meshheading:2137089-Nucleoplasmins, pubmed-meshheading:2137089-Oncogene Proteins, Viral, pubmed-meshheading:2137089-Peptides, pubmed-meshheading:2137089-Phosphoproteins, pubmed-meshheading:2137089-Protein Sorting Signals, pubmed-meshheading:2137089-Repressor Proteins, pubmed-meshheading:2137089-Signal Transduction

Comparison of diverse transport signals in synthetic peptide-induced nuclear transport.

Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S.