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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2011-3-3
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pubmed:databankReference |
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pubmed:abstractText |
Bacillus subtilis BCC41051 producing a thermostable ?-mannanase was isolated from soybean meal-enriched soil and was unexpectedly found to be thermophilic in nature. The extracellular ?-mannanase (ManA) produced was hydrophilic, as it was not precipitated even with ammonium sulfate at 80% saturation. The estimated molecular weight of ManA was 38.0 kDa by SDS-PAGE with a pi value of 5.3. Optimal pH and temperature for mannan-hydrolyzing activity was 7.0 and 60°C, respectively. The enzyme was stable over a pH range of 5.0-11.5, and at temperatures of up to 60°C for 30 min, with more than 80% of its activity retained. ManA was strongly inhibited by Hg(2+) (1 mM), but was sensitive to other divalent ions to a lesser degree. The gene of ManA encoded a protein of 362 amino acid residues, with the first 26 residues identified as a signal peptide. High expression of recombinant ManA was achieved in both Escherichia coli BL21 (DE3) (415.18 U/ml) and B. megaterium UNcat (359 U/ml).
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
1976-3794
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
49
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
86-93
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pubmed:meshHeading |
pubmed-meshheading:21369984-Bacillus megaterium,
pubmed-meshheading:21369984-Bacillus subtilis,
pubmed-meshheading:21369984-Cations, Divalent,
pubmed-meshheading:21369984-Chemical Fractionation,
pubmed-meshheading:21369984-Cloning, Molecular,
pubmed-meshheading:21369984-DNA, Bacterial,
pubmed-meshheading:21369984-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:21369984-Enzyme Inhibitors,
pubmed-meshheading:21369984-Enzyme Stability,
pubmed-meshheading:21369984-Escherichia coli,
pubmed-meshheading:21369984-Gene Expression,
pubmed-meshheading:21369984-Hydrogen-Ion Concentration,
pubmed-meshheading:21369984-Hydrophobic and Hydrophilic Interactions,
pubmed-meshheading:21369984-Isoelectric Point,
pubmed-meshheading:21369984-Mercury,
pubmed-meshheading:21369984-Molecular Sequence Data,
pubmed-meshheading:21369984-Molecular Weight,
pubmed-meshheading:21369984-Protein Sorting Signals,
pubmed-meshheading:21369984-Sequence Analysis, DNA,
pubmed-meshheading:21369984-Soil Microbiology,
pubmed-meshheading:21369984-Temperature,
pubmed-meshheading:21369984-beta-Mannosidase
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pubmed:year |
2011
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pubmed:articleTitle |
Characterization, gene cloning, and heterologous expression of ?-mannanase from a thermophilic Bacillus subtilis.
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pubmed:affiliation |
Department of Biotechnology, Mahidol University, Rama VI Rd., Bangkok, 10400, Thailand.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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