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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1990-2-21
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pubmed:abstractText |
The mechanism of transcriptional regulation of the H-2Kbm1 major histocompatibility complex (MHC) class I gene by adenovirus type 12 E1A (Ad12-E1A) was studied in transfected rat embryonal fibroblasts. Results of long-term expression of the chloramphenicol acetyl transferase (CAT) gene placed under the control of the 5'-flanking region of the mouse MHC class I gene. H-2Kbm1, and the results of nuclear run-on transcription assays, yield evidence for both positive and negative regulation of H-2Kbm1 by E1A gene product. Deletion studies in the H-2Kbm1 promoter region revealed that a proximal 58 bp upstream sequence (-194 to -136, relative to the cap site) and a distal 316 bp sequence (-1837 to -1521) respectively contribute to positive and negative regulation mediated by the E1A gene product. Both regulatory elements of MHC class I gene promoter region are responsible for the differential expression of the H-2Kbm1 gene in Ad12 transformed cells. A nuclear factor binding to the negative element has been detected only in extracts derived from cells expressing Ad12-E1A.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2136829-166944,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2136829-222468,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2136829-2444981,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2136829-2484015,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2136829-271968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2136829-2832733,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/2136829-2963331,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2136829-2968712,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2136829-2982503,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2136829-2989693,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2136829-2999984,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2136829-3011403,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2136829-3022137,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2136829-3025599,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/2136829-3467324,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2136829-3475569,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2136829-3510743,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2136829-356353,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2136829-3595566,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2136829-3748155,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2136829-3823900,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2136829-4075400,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/2136829-6152713,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/2136829-6527689,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2136829-6828150,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2136829-6828386,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2136829-6933049,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2136829-6960240,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2136829-92183
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0261-4189
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
9
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
127-35
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2136829-Humans,
pubmed-meshheading:2136829-Animals,
pubmed-meshheading:2136829-Cell Transformation, Neoplastic,
pubmed-meshheading:2136829-Base Sequence,
pubmed-meshheading:2136829-RNA, Messenger,
pubmed-meshheading:2136829-Cell Line,
pubmed-meshheading:2136829-Cell Transformation, Viral,
pubmed-meshheading:2136829-Molecular Sequence Data,
pubmed-meshheading:2136829-Transcription, Genetic,
pubmed-meshheading:2136829-H-2 Antigens,
pubmed-meshheading:2136829-Gene Expression Regulation,
pubmed-meshheading:2136829-Genes, MHC Class I,
pubmed-meshheading:2136829-Promoter Regions, Genetic,
pubmed-meshheading:2136829-Transfection,
pubmed-meshheading:2136829-Regulatory Sequences, Nucleic Acid
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