Linked Life Data

21359962

Source:http://linkedlifedata.com/resource/pubmed/id/21359962

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PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2011-4-19
pubmed:abstractText
Huang Qi Huai (HQH) granules, a mixture of Chinese herbs, contains trametes robiniophila murr, wolfberry fruit, and Polygonatum. We investigated the mechanism of the protective effects of HQH on adriamycin nephrosis (ADR) in rats. Adriamycin nephrotic rats were induced by a single dose of 5 mg/kg adriamycin. For the HQH-treated adriamycin nephrosis group, 1 day after treatment with 5 mg/kg adriamycin, the rats were administered once-daily oral gavage of 2 mg/kg HQH for 15 days. All the rats were killed at day 15. Histological changes were observed by light microscopy and transmission electron microscope. Nephrin and podocin expression levels were measured by real-time RT-PCR and Western blot. Proteinuria was measured by the Bradford protein assay. Serum TNF-? and IL-1? levels were evaluated by ELISA. Macrophage infiltration was detected by immunohistochemistry and immunoblotting, respectively. ADR rats showed heavy proteinuria, podocyte and tubulointerstitial injury, macrophage infiltration, and increased levels of serum cytokines TNF-? and IL-1?. HQH significantly ameliorated the adriamycin-induced renal injury. These data were validated in the cultured podocytes. The podocytes were treated by adriamycin in the presence or absence of HQH and nephrin and podocin expression and TNF-? and IL-1? synthesis and secretion were determined by real-time RT-PCR, immunoblotting, and ELISA, respectively. Adriamycin significantly reduced nephrin and podocin expression, which was significantly restored by the treatment of HQH. HQH treatment inhibited adriamycin-induced TNF-? and IL-1? expression. Our findings suggest that HQH significantly reduces proteinuria, prevents podocyte injury, and ameliorates tubulointerstitial damage. Inhibition of inflammatory cytokine expression and macrophage infiltration may be the protective mechanism of HQH.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1432-198X
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
26
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
905-13
pubmed:meshHeading
pubmed-meshheading:21359962-Administration, Oral, pubmed-meshheading:21359962-Animals, pubmed-meshheading:21359962-Antibiotics, Antineoplastic, pubmed-meshheading:21359962-Cell Line, Transformed, pubmed-meshheading:21359962-Clinical Chemistry Tests, pubmed-meshheading:21359962-Disease Models, Animal, pubmed-meshheading:21359962-Doxorubicin, pubmed-meshheading:21359962-Drugs, Chinese Herbal, pubmed-meshheading:21359962-Gene Expression, pubmed-meshheading:21359962-Interleukin-1beta, pubmed-meshheading:21359962-Intracellular Signaling Peptides and Proteins, pubmed-meshheading:21359962-Kidney, pubmed-meshheading:21359962-Macrophages, pubmed-meshheading:21359962-Membrane Proteins, pubmed-meshheading:21359962-Mice, pubmed-meshheading:21359962-Nephrosis, pubmed-meshheading:21359962-Phytotherapy, pubmed-meshheading:21359962-Podocytes, pubmed-meshheading:21359962-Proteinuria, pubmed-meshheading:21359962-RNA, Messenger, pubmed-meshheading:21359962-Rats, pubmed-meshheading:21359962-Rats, Sprague-Dawley, pubmed-meshheading:21359962-Tumor Necrosis Factor-alpha
pubmed:year
2011
pubmed:articleTitle
Protective effects of Huang Qi Huai granules on adriamycin nephrosis in rats.
pubmed:affiliation
Department of Nephrology, Nanjing Children's Hospital, Nanjing Medical University, Nanjing, 210008, Jiangsu Province, China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't