Linked Life Data

21356388

Source:http://linkedlifedata.com/resource/pubmed/id/21356388

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2011-3-1
pubmed:abstractText
Cancer-associated fibroblasts expressing fibroblast activation protein (FAP) have been implicated in the invasive behavior of colorectal cancer. In this study, we use FAP expression as a marker of fibroblast activation and analyze the effect of activated fibroblasts on colorectal cancer migration and invasion in experimental cell studies. We also investigated the expression pattern of FAP in cancer-associated fibroblasts during transformation from benign to malignant colorectal tumors. In immunohistochemical analyses, FAP was expressed in fibroblasts in all colorectal cancer samples examined, whereas all normal colon, hyperplastic polyps, or adenoma samples were negative. In in vitro studies, conditioned medium from colon cancer cells, but not adenoma cells, activated fibroblasts by inducing FAP expression. These activated fibroblasts increased the migration and invasion of colon cancer cells in Boyden chamber experiments and in a three-dimensional cell culture model. We identify fibroblast growth factor 1/fibroblast growth factor receptor 3 (FGF1/FGFR-3) signaling as mediators leading to the increased migration and invasion. Activated fibroblasts increase their expression of FGF1, and by adding a fibroblast growth factor receptor inhibitor, as well as an FGF1-neutralizing antibody, we reduced the migration of colon cancer cells. Our findings provide evidence of a possible molecular mechanism involved in the cross talk between cancer cells and fibroblasts leading to cancer cell invasion.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
1525-2191
pubmed:author
pubmed:copyrightInfo
Copyright © 2011 American Society for Investigative Pathology. Published by Elsevier Inc. All rights reserved.
pubmed:issnType
Electronic
pubmed:volume
178
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1387-94
pubmed:meshHeading
pubmed-meshheading:21356388-Actins, pubmed-meshheading:21356388-Cell Culture Techniques, pubmed-meshheading:21356388-Cell Line, Tumor, pubmed-meshheading:21356388-Cell Movement, pubmed-meshheading:21356388-Cell Shape, pubmed-meshheading:21356388-Colorectal Neoplasms, pubmed-meshheading:21356388-Culture Media, Conditioned, pubmed-meshheading:21356388-Fibroblast Growth Factor 1, pubmed-meshheading:21356388-Fibroblasts, pubmed-meshheading:21356388-Gelatinases, pubmed-meshheading:21356388-Humans, pubmed-meshheading:21356388-Membrane Proteins, pubmed-meshheading:21356388-Models, Biological, pubmed-meshheading:21356388-Neoplasm Invasiveness, pubmed-meshheading:21356388-Receptor, Fibroblast Growth Factor, Type 3, pubmed-meshheading:21356388-Serine Endopeptidases, pubmed-meshheading:21356388-Signal Transduction
pubmed:year
2011
pubmed:articleTitle
Colorectal cancer cells activate adjacent fibroblasts resulting in FGF1/FGFR3 signaling and increased invasion.
pubmed:affiliation
Department of Medical Biosciences, Pathology, Umeå University, Umeå, Sweden. maria.henriksson@medbio.umu.se
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't