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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2011-4-19
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pubmed:abstractText |
The aim of this study was to test the hypothesis that nuclear factor-kappa B (NF-?B) is involved in TRPV4-NO pathway in thermal hyperalgesia following chronic compression of the dorsal root ganglion (DRG) (the procedure hereafter termed CCD) in rat. Intrathecal administration of two NF-?B inhibitors, pyrrolidine dithiocarbamate (PDTC; 10(-1) to 10(-2)M) and BAY (100-50 ?M), both induced significantly dose-dependent increase in the paw withdrawal latency (PWL) and decrease in nitric oxide (NO) content in DRG when compared with control rats. Pretreatment with 4?-phorbol 12,13-didecanoate (4?-PDD, transient receptor potential vanilloid 4 (TRPV4) synthetic activator, 1 nm) attenuated the suppressive effects of PDTC (10(-1)M) and BAY (100 ?M) on CCD-induced thermal hyperalgesia and NO production. In addition, Western blot analysis indicated that CCD rats exhibited nuclear NF-?B protein expression and low levels of cytoplasmic inhibitory-kappa B (I-?B) expression; the increase in NF-?B expression and decrease in I-?B expression were reversed after intrathecal injection of PDTC. In conclusion, our data suggested that NF-?B could be involved in TRPV4-NO pathway in CCD-induced thermal hyperalgesia.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/3-(4-methylphenylsulfonyl)-2-propene...,
http://linkedlifedata.com/resource/pubmed/chemical/4alpha-phorbol 12,13-didecanone,
http://linkedlifedata.com/resource/pubmed/chemical/Analgesics,
http://linkedlifedata.com/resource/pubmed/chemical/I-kappa B Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/NF-kappa B,
http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide,
http://linkedlifedata.com/resource/pubmed/chemical/Nitriles,
http://linkedlifedata.com/resource/pubmed/chemical/Phorbols,
http://linkedlifedata.com/resource/pubmed/chemical/Pyrrolidines,
http://linkedlifedata.com/resource/pubmed/chemical/Sulfones,
http://linkedlifedata.com/resource/pubmed/chemical/TRPV Cation Channels,
http://linkedlifedata.com/resource/pubmed/chemical/Thiocarbamates,
http://linkedlifedata.com/resource/pubmed/chemical/Trpv4 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/pyrrolidine dithiocarbamic acid
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
1872-7549
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pubmed:author |
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pubmed:copyrightInfo |
Copyright © 2011 Elsevier B.V. All rights reserved.
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pubmed:issnType |
Electronic
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pubmed:day |
1
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pubmed:volume |
221
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
19-24
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pubmed:meshHeading |
pubmed-meshheading:21356247-Analgesics,
pubmed-meshheading:21356247-Animals,
pubmed-meshheading:21356247-Disease Models, Animal,
pubmed-meshheading:21356247-Dose-Response Relationship, Drug,
pubmed-meshheading:21356247-Ganglia, Spinal,
pubmed-meshheading:21356247-Hyperalgesia,
pubmed-meshheading:21356247-I-kappa B Proteins,
pubmed-meshheading:21356247-Male,
pubmed-meshheading:21356247-NF-kappa B,
pubmed-meshheading:21356247-Nerve Compression Syndromes,
pubmed-meshheading:21356247-Neuralgia,
pubmed-meshheading:21356247-Nitric Oxide,
pubmed-meshheading:21356247-Nitriles,
pubmed-meshheading:21356247-Phorbols,
pubmed-meshheading:21356247-Pyrrolidines,
pubmed-meshheading:21356247-Rats,
pubmed-meshheading:21356247-Rats, Wistar,
pubmed-meshheading:21356247-Signal Transduction,
pubmed-meshheading:21356247-Sulfones,
pubmed-meshheading:21356247-TRPV Cation Channels,
pubmed-meshheading:21356247-Thiocarbamates
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pubmed:year |
2011
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pubmed:articleTitle |
Nuclear factor-kappa B mediates TRPV4-NO pathway involved in thermal hyperalgesia following chronic compression of the dorsal root ganglion in rats.
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pubmed:affiliation |
Department of Physical Medicine & Rehabilitation, Qilu Hospital, Medical School of Shandong University, Jinan 250012, China.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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