Linked Life Data

21354137

Source:http://linkedlifedata.com/resource/pubmed/id/21354137

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2011-4-25
pubmed:abstractText
Human fetal eyes 8-40 weeks gestation (WG) were examined using markers to hematopoietic stem cells (HSC), vascular precursor cells (VPC), monocytes/macrophages and endothelial cells (EC). Electron microscopy and bromo-deoxyuridene labeling were undertaken to confirm the existence of solid vascular cords and to demonstrate vasculogenesis and angiogenesis in developing choroidal tissue. Our results demonstrated that the earliest incipient choroid consisted of vimentin(+) mesenchymal precursor cells which downregulated vimentin expression with maturation. Our observations lead us to conclude that these vimentin(-)/CD34(+)/CD44(+)/CD133(+) HSCs then differentiated into three distinct lineages: single isolated CD34(-)/CD39(+) VPCs that formed solid vascular cords which lumenized and became lined with CD34(+) vascular ECs; CD34(--+)/CD14(+)/CD68(+) monocytes that differentiated into tissue macrophages; and CD133(+)/CD34(--+)/?-smooth muscle actin(+) mural precursor cells that matured into smooth muscle cells and pericytes. Blood vessel formation occurred throughout the whole choroid simultaneously, indicative of in situ differentiation. Vasculogenesis, as evidenced by lumenization of solid vascular cords, was responsible for the formation of the entire choroidal area with angiogenesis, in all three layers of the choroid, only adding to vascular density. These results suggest that formation of the human choroid involves three processes: HSC differentiation, vasculogenesis and angiogenesis. Since vasculogenesis takes place independently of VEGF(165), further insights regarding the molecular mechanisms of vasculogenesis are required to better inform future treatments of choroidal neovascularization.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1096-0007
pubmed:author
pubmed:copyrightInfo
Crown Copyright © 2011. Published by Elsevier Ltd. All rights reserved.
pubmed:issnType
Electronic
pubmed:volume
92
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
361-76
pubmed:dateRevised
2011-7-15
pubmed:meshHeading
pubmed-meshheading:21354137-Actins, pubmed-meshheading:21354137-Antigens, CD, pubmed-meshheading:21354137-Biological Markers, pubmed-meshheading:21354137-Capillaries, pubmed-meshheading:21354137-Cell Differentiation, pubmed-meshheading:21354137-Cell Lineage, pubmed-meshheading:21354137-Choroid, pubmed-meshheading:21354137-Endothelium, Vascular, pubmed-meshheading:21354137-Gestational Age, pubmed-meshheading:21354137-Hematopoietic Stem Cells, pubmed-meshheading:21354137-Humans, pubmed-meshheading:21354137-Immunohistochemistry, pubmed-meshheading:21354137-Ki-67 Antigen, pubmed-meshheading:21354137-Macrophages, pubmed-meshheading:21354137-Mesenchymal Stem Cells, pubmed-meshheading:21354137-Microscopy, Confocal, pubmed-meshheading:21354137-Microscopy, Electron, pubmed-meshheading:21354137-Neovascularization, Physiologic, pubmed-meshheading:21354137-Vimentin
pubmed:year
2011
pubmed:articleTitle
Evidence of hematopoietic differentiation, vasculogenesis and angiogenesis in the formation of human choroidal blood vessels.
pubmed:affiliation
Department of Anatomy, Bosch Institute, University of Sydney, Sydney, NSW 2006, Australia. tailoi@anatomy.usyd.edu.au
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't