Source:http://linkedlifedata.com/resource/pubmed/id/21353767
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
2011-3-22
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pubmed:abstractText |
In this work, an electrochemiluminescence (ECL) immunosensor for ultrasensitive detection of ?-fetoprotein (AFP) was fabricated using Ru(bpy)(3)(2+)-encapsulated liposome as the label and electrodeposited gold nanoparticles (GNPs) as the immobilizing support. Great signal amplification was achieved since liposome could encapsulate large amount of reporter molecules and GNPs could provide large active surface. Under optimized conditions, with sandwich type format, a linear range of AFP from 0.005 to 0.2 pg/mL and an extremely low detection limit of 0.001 pg/mL was obtained, much lower than that in previous reports. The proposed ECL immnuosensor showed high sensitivity, specificity, and good stability, which may open a new door to ultrasensitive detection of proteins in clinical analysis.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/2,2'-Dipyridyl,
http://linkedlifedata.com/resource/pubmed/chemical/Capsules,
http://linkedlifedata.com/resource/pubmed/chemical/Gold,
http://linkedlifedata.com/resource/pubmed/chemical/Liposomes,
http://linkedlifedata.com/resource/pubmed/chemical/Organometallic Compounds,
http://linkedlifedata.com/resource/pubmed/chemical/alpha-Fetoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/ruthenium (bpy)3(2 )-C2O4(2-)
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
1873-4367
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pubmed:author | |
pubmed:copyrightInfo |
Copyright © 2011 Elsevier B.V. All rights reserved.
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pubmed:issnType |
Electronic
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pubmed:day |
1
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pubmed:volume |
84
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
515-9
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:21353767-2,2'-Dipyridyl,
pubmed-meshheading:21353767-Biosensing Techniques,
pubmed-meshheading:21353767-Capsules,
pubmed-meshheading:21353767-Gold,
pubmed-meshheading:21353767-Limit of Detection,
pubmed-meshheading:21353767-Liposomes,
pubmed-meshheading:21353767-Luminescent Measurements,
pubmed-meshheading:21353767-Nanoparticles,
pubmed-meshheading:21353767-Organometallic Compounds,
pubmed-meshheading:21353767-alpha-Fetoproteins
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pubmed:year |
2011
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pubmed:articleTitle |
Electrochemiluminescence immunosensor for ?-fetoprotein using Ru(bpy)3(2+)-encapsulated liposome as labels.
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pubmed:affiliation |
Anhui Key Laboratory of Chemo-biosensing, College of Chemistry and Materials Science, Anhui Normal University, Wuhu 241000, PR China. hywang@mail.ahnu.edu.cn
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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