Linked Life Data

21348637

Source:http://linkedlifedata.com/resource/pubmed/id/21348637

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2011-5-11
pubmed:abstractText
Although estrogen receptors (ERs) are expressed in human hepatocellular carcinoma (HCC), several clinical trials have failed to demonstrate the efficacy of antiestrogen treatment in HCC patients. Recently, the identification of several ER splicing variants has enlightened the complex nature of estrogen signaling in peripheral tissues; this may help understanding estrogen role in either nontumoral or malignant nonclassical target organs, including liver. In this work we have investigated mRNA expression of wild-type and splice variants of ER? in nontumoral, cirrhotic, and malignant human liver, as well as in HCC cell lines, using an exon-specific reverse transcription polymerase chain reaction (RT-PCR). In particular, ER?66 was detected in nontumoral and, to a lesser extent, in cirrhotic liver tissues, whereas its expression decreased or became undetectable in HCC tissues and cell lines. The ER?46 splicing variant was detected ubiquitously in all samples; interestingly, however, the ER?36 variant was inversely expressed with respect to ER?66, being highest in HepG2 cells, intermediate in Huh7 cells, and lowest in HA22T cells. It is noteworthy that aromatase was correspondingly expressed with ER?36 and inversely related to ER?66. This observation suggests that a switch from ER?66 to a predominant expression of ER?36 may be associated with development and/or progression of human HCC.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1557-8100
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
15
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
313-7
pubmed:meshHeading
pubmed:year
2011
pubmed:articleTitle
Expression of wild-type and variant estrogen receptor alpha in liver carcinogenesis and tumor progression.
pubmed:affiliation
Experimental Oncology, Department of Oncology, ARNAS-Civico, Palermo, Italy.
pubmed:publicationType
Journal Article