Linked Life Data

21346156

Source:http://linkedlifedata.com/resource/pubmed/id/21346156

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2011-5-27
pubmed:abstractText
Glioblastoma multiforme (GBM) is the most common and aggressive of the primary brain tumors. These tumors express multiple members of the epithelial sodium channel (ENaC)/degenerin (Deg) family and are associated with a basally active amiloride-sensitive cation current. We hypothesize that this glioma current is mediated by a hybrid channel composed of a mixture of ENaC and acid-sensing ion channel (ASIC) subunits. To test the hypothesis that ASIC1 interacts with ?ENaC and ?ENaC at the cellular level, we have used total internal reflection fluorescence microscopy (TIRFM) in live rat astrocytes transiently cotransfected with cDNAs for ASIC1-DsRed plus ?ENaC-yellow fluorescent protein (YFP) or ASIC1-DsRed plus ?ENaC-YFP. TIRFM images show colocalization of ASIC1 with both ?ENaC and ?ENaC. Furthermore, using TIRFM in stably transfected D54-MG cells, we also found that ASIC1 and ?ENaC both localize to a submembrane region following exposure to pH 6.0, similar to the acidic conditions found in the core of a glioblastoma lesion. Using high-resolution clear native gel electrophoresis, we found that ASIC1 forms a complex with ENaC subunits which migrates at ?480 kDa in D54-MG glioma cells. These data suggest that different ENaC/Deg subunits interact and could combine to form a hybrid channel that likely underlies the amiloride-sensitive current seen in human glioma cells.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1522-1563
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
300
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
C1246-59
pubmed:meshHeading
pubmed:year
2011
pubmed:articleTitle
Interaction of ASIC1 and ENaC subunits in human glioma cells and rat astrocytes.
pubmed:affiliation
Dept. of Physiology and Biophysics, University of Alabama at Birmingham, 1918 University Blvd., Birmingham, AL 35294, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, N.I.H., Extramural