Linked Life Data

21345559

Source:http://linkedlifedata.com/resource/pubmed/id/21345559

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2011-4-8
pubmed:abstractText
Currently HPLC/MS is the state of the art tool for environmental/drinking water perfluorooctane sulfonate (PFOS) monitoring. PFOS can bind to peroxisomal proliferator-activated receptor-alpha (PPAR?), which forms heterodimers with retinoid X receptors (RXRs) and binds to PPAR response elements. In this bioassay free PFOS in water samples competes with immobilized PFOS in ELISA plates for a given amount of PPAR?-RXR?. It can be determined indirectly by immobilizing PPAR?-RXR?-PFOS complex to another plate coated with PPAR? antibody and subsequent measuring the level of PPAR?-RXR? by using biotin-modified PPAR?-RXR? probes-quantum dots-streptavidin detection system. The rapid and high-throughput bioassay demonstrated a detection limit of 2.5 ng L(-1) with linear range between 2.5 ng L(-1) and 75 ng L(-1). Detection results of environmental water samples were highly consistent between the bioassay and HPLC/MS.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1873-6424
pubmed:author
pubmed:copyrightInfo
Copyright © 2011 Elsevier Ltd. All rights reserved.
pubmed:issnType
Electronic
pubmed:volume
159
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1348-53
pubmed:meshHeading
pubmed:year
2011
pubmed:articleTitle
A rapid and high-throughput quantum dots bioassay for monitoring of perfluorooctane sulfonate in environmental water samples.
pubmed:affiliation
Minister of Education Key Laboratory of Environment and Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei Province 430030, PR China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Validation Studies