Source:http://linkedlifedata.com/resource/pubmed/id/21344498
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
7
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| pubmed:dateCreated |
2011-5-13
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| pubmed:abstractText |
The dynamics between inflammatory factors, mechanical stress, and healing factors, in an intra-articular joint, are very complex after injury. Injury to intra-articular tissue [anterior cruciate ligament (ACL), synovium] results in hypoxia, accumulation of various pro-inflammatory factors, cytokines, and metalloproteases. Although the presence of increased amounts of matrix-metalloproteinases (MMP) in the joint fluid after knee injury is considered the key factor for ACL poor healing ability; however, the exact role of collective participants of the joint fluid on MMP-2 activity and production has not been fully studied yet. To investigate the combined effects of mechanical injury, inflammation and hypoxia induced factor-1? (HIF-1?) on induction of MMP-2; we mimicked the microenvironment of joint cavity after ACL injury. The results show that TNF-? and IL-1? elevate the activity of MMP-2 in a dose- and time-dependent manner. In addition, mechanical stretch further enhances the MMP-2 protein levels with TNF-?, IL-1?, and their mixture. CoCl(2) -induced HIF-1? (100 and 500?µM) also increases the levels and activity of MMP-2. Mechanical stretch has a strong additional effect on MMP-2 production with HIF-1?. Our results conclude that mechanical injury, HIF-1? and inflammatory factors collectively induce increased MMP-2 production in ACL fibroblasts, which was inhibited by NF-?B pathway inhibitor (Bay-11-7082).
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/3-(4-methylphenylsulfonyl)-2-propene...,
http://linkedlifedata.com/resource/pubmed/chemical/Cobalt,
http://linkedlifedata.com/resource/pubmed/chemical/HIF1A protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Hypoxia-Inducible Factor 1, alpha...,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-1beta,
http://linkedlifedata.com/resource/pubmed/chemical/MMP2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Matrix Metalloproteinase 2,
http://linkedlifedata.com/resource/pubmed/chemical/NF-kappa B,
http://linkedlifedata.com/resource/pubmed/chemical/Nitriles,
http://linkedlifedata.com/resource/pubmed/chemical/Sulfones,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factor-alpha,
http://linkedlifedata.com/resource/pubmed/chemical/cobaltous chloride
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| pubmed:status |
MEDLINE
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| pubmed:month |
Jul
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| pubmed:issn |
1554-527X
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| pubmed:author | |
| pubmed:copyrightInfo |
Copyright © 2011 Orthpaedic Research Society.
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| pubmed:issnType |
Electronic
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| pubmed:volume |
29
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
1008-14
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| pubmed:meshHeading |
pubmed-meshheading:21344498-Adult,
pubmed-meshheading:21344498-Anoxia,
pubmed-meshheading:21344498-Anterior Cruciate Ligament,
pubmed-meshheading:21344498-Cells, Cultured,
pubmed-meshheading:21344498-Cobalt,
pubmed-meshheading:21344498-Drug Synergism,
pubmed-meshheading:21344498-Fibroblasts,
pubmed-meshheading:21344498-Humans,
pubmed-meshheading:21344498-Hypoxia-Inducible Factor 1, alpha Subunit,
pubmed-meshheading:21344498-Inflammation,
pubmed-meshheading:21344498-Interleukin-1beta,
pubmed-meshheading:21344498-Matrix Metalloproteinase 2,
pubmed-meshheading:21344498-Middle Aged,
pubmed-meshheading:21344498-NF-kappa B,
pubmed-meshheading:21344498-Nitriles,
pubmed-meshheading:21344498-Stress, Mechanical,
pubmed-meshheading:21344498-Sulfones,
pubmed-meshheading:21344498-Tumor Necrosis Factor-alpha,
pubmed-meshheading:21344498-Young Adult
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| pubmed:year |
2011
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| pubmed:articleTitle |
Combined effects of TNF-?, IL-1?, and HIF-1? on MMP-2 production in ACL fibroblasts under mechanical stretch: an in vitro study.
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| pubmed:affiliation |
Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Bioengineering College, Chongqing University, Chongqing 400044, PR China.
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| pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, Non-U.S. Gov't
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