Source:http://linkedlifedata.com/resource/pubmed/id/21342606
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
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| pubmed:dateCreated |
2011-7-13
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| pubmed:abstractText |
The present study determined whether ?-ketoglutarate (AKG) might affect the expression of AMP-activated protein kinase (AMPK) and energy status in the intestinal mucosa of piglets challenged with Escherichia coli lipopolysaccharide (LPS). A total of eighteen piglets (weaned at 21 d of age) were allocated to one of three treatments: (1) non-challenged (control); (2) LPS-challenged (LPS); (3) LPS+1 % AKG (LPS+AKG). Piglets in the control and LPS groups were fed a maize- and soyabean meal-based diet, and the LPS+AKG group was fed the basal diet supplemented with 1 % AKG. On days 10, 12, 14 and 16 of the trial, piglets in the LPS and LPS+AKG groups were challenged with LPS (80 ?g/kg body weight), whereas piglets in the control group received the same volume of sterile saline. Pigs were euthanised 24 h after the last administration of LPS or saline to obtain intestinal mucosae for biochemical analysis. Compared with the control group, LPS administration decreased (P < 0·05) the oxidation of AKG, oleic acid, glutamine and glucose in enterocytes, decreased concentrations of ATP in the duodenal and jejunal mucosae and decreased adenylate energy charge (AMP:ATP ratio) in the jejunal and ileal mucosae. Additionally, LPS treatment reduced (P < 0·05) mucosal concentrations of phosphorylated AMPK in the jejunum and ileum as well as acetyl-CoA carboxylase in all segments of the small intestine. The adverse effects of LPS were reversed by AKG. Collectively, these results indicate that dietary supplementation with 1 % AKG beneficially modulates the AMPK signalling pathway to improve energy status in the small intestine of LPS-challenged piglets.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/AMP-Activated Protein Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Acetyl-CoA Carboxylase,
http://linkedlifedata.com/resource/pubmed/chemical/Adenosine Monophosphate,
http://linkedlifedata.com/resource/pubmed/chemical/Adenosine Triphosphate,
http://linkedlifedata.com/resource/pubmed/chemical/Glucose,
http://linkedlifedata.com/resource/pubmed/chemical/Glutamine,
http://linkedlifedata.com/resource/pubmed/chemical/Ketoglutaric Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Lipopolysaccharides,
http://linkedlifedata.com/resource/pubmed/chemical/Oleic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/alpha-ketoglutaric acid
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| pubmed:status |
MEDLINE
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| pubmed:month |
Aug
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| pubmed:issn |
1475-2662
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| pubmed:author |
pubmed-author:DingBinyingB,
pubmed-author:FastM LML,
pubmed-author:HaleC WCW,
pubmed-author:HouYongqingY,
pubmed-author:KangPingP,
pubmed-author:LiYongtangY,
pubmed-author:LiuJianJ,
pubmed-author:LiuYulanY,
pubmed-author:WangLeiL,
pubmed-author:WuGuoyaoG,
pubmed-author:YinYulongY,
pubmed-author:ZhuHuilingH
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| pubmed:issnType |
Electronic
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| pubmed:volume |
106
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
357-63
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| pubmed:meshHeading |
pubmed-meshheading:21342606-AMP-Activated Protein Kinases,
pubmed-meshheading:21342606-Acetyl-CoA Carboxylase,
pubmed-meshheading:21342606-Adenosine Monophosphate,
pubmed-meshheading:21342606-Adenosine Triphosphate,
pubmed-meshheading:21342606-Animals,
pubmed-meshheading:21342606-Dietary Supplements,
pubmed-meshheading:21342606-Enterocytes,
pubmed-meshheading:21342606-Escherichia coli,
pubmed-meshheading:21342606-Female,
pubmed-meshheading:21342606-Glucose,
pubmed-meshheading:21342606-Glutamine,
pubmed-meshheading:21342606-Intestinal Diseases,
pubmed-meshheading:21342606-Intestinal Mucosa,
pubmed-meshheading:21342606-Intestine, Small,
pubmed-meshheading:21342606-Ketoglutaric Acids,
pubmed-meshheading:21342606-Lipopolysaccharides,
pubmed-meshheading:21342606-Oleic Acid,
pubmed-meshheading:21342606-Oxidation-Reduction,
pubmed-meshheading:21342606-Phosphorylation,
pubmed-meshheading:21342606-Signal Transduction,
pubmed-meshheading:21342606-Swine,
pubmed-meshheading:21342606-Weaning
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| pubmed:year |
2011
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| pubmed:articleTitle |
Effects of ?-ketoglutarate on energy status in the intestinal mucosa of weaned piglets chronically challenged with lipopolysaccharide.
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| pubmed:affiliation |
Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, People's Republic of China. houyq777@yahoo.com.cn
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| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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