Source:http://linkedlifedata.com/resource/pubmed/id/21339898
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:dateCreated |
2011-2-22
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| pubmed:abstractText |
Tooth and bone specimen require extensive demineralization for careful analysis of cell morphology, as well as gene and protein expression levels. The LacZ gene, which encodes the ß-galactosidase enzyme, is often used as a reporter gene to study gene-structure function, tissue-specific expression by a promoter, cell lineage and fate. This reporter gene is particularly useful for analyzing the spatial and temporal gene expression pattern, by expressing the LacZ gene under the control of a promoter of interest. To analyze LacZ activity, and the expression of other genes and their protein products in teeth and bones, it is necessary to carry out a complete demineralization of the specimen before cutting sections. However, strong acids, such as formic acid used for tooth demineralization, destroy the activities of enzymes including those of ß-galactosidase. Therefore, most protocols currently use mild acids such as 0.1 M ethylene diamine tetra-acetic acid (EDTA) for demineralization of tooth and bone specimen, which require a longer period of treatment for complete demineralization. A method by which hard tissue specimens such as teeth and bones can be rapidly, but gently, decalcified is necessary to save time and effort. Here, we report a suitable method for rapid demineralization of mouse teeth in 0.1M EDTA at 42?C without any loss of ß-galactosidase activity.
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| pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/21339898-10191045,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21339898-10610024,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21339898-10817669,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21339898-10998258,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21339898-11596012,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21339898-12364575,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21339898-12533791,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21339898-12721295,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21339898-16098949,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21339898-19348940,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21339898-19379665,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21339898-2108204,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21339898-2120156,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21339898-4861607,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21339898-7626404,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21339898-7689084,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21339898-9545272,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21339898-9562383
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:status |
PubMed-not-MEDLINE
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| pubmed:issn |
1874-2106
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| pubmed:author | |
| pubmed:issnType |
Electronic
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| pubmed:volume |
4
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
223-9
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| pubmed:dateRevised |
2011-7-25
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| pubmed:year |
2010
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| pubmed:articleTitle |
A method for rapid demineralization of teeth and bones.
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| pubmed:affiliation |
Gene Targeting Facility, Laboratory of Cell and Developmental Biology, National Institute of Dental and Craniofacial Research, National Institutes of Health, 30 Convent Drive, Bethesda, MD 20892, USA.
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| pubmed:publicationType |
Journal Article
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