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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
2011-2-21
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pubmed:abstractText |
Posttraumatic arthritis commonly develops following articular fracture. The objective of this study was to develop a closed joint model of transarticular impact with and without creation of an articular fracture that maintains the physiologic environment during loading. Fresh intact porcine knees were preloaded and impacted at 294 J via a drop track. Osteochondral cores were obtained from the medial and lateral aspects of the femoral condyles and tibial plateau. Chondrocyte viability was assessed at days 0, 3, and 5 postimpact in sham, impacted nonfractured, and impacted fractured joints. Total matrix metalloproteinase (MMP) activity, aggrecanase (ADAMTS-4) activity, and sulfated glycosaminoglycan (S-GAG) release were measured in culture media from days 3 and 5 posttrauma. No differences were observed in chondrocyte viability of impacted nonfractured joints (95.9 ± 6.9%) when compared to sham joints (93.8 ± 7.7%). In impacted fractured joints, viability of the fractured edge was 40.5 ± 27.6% and significantly lower than all other sites, including cartilage adjacent to the fractured edge (p < 0.001). MMP and aggrecanase activity and S-GAG release were significantly increased in specimens from the fractured edge. This study showed that joint impact resulting in articular fracture significantly decreased chondrocyte viability, increased production of MMPs and aggrecanases, and enhanced S-GAG release, whereas the same level of impact without fracture did not cause such changes.
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pubmed:grant |
http://linkedlifedata.com/resource/pubmed/grant/AG15768,
http://linkedlifedata.com/resource/pubmed/grant/AR055659,
http://linkedlifedata.com/resource/pubmed/grant/AR48182,
http://linkedlifedata.com/resource/pubmed/grant/AR48852,
http://linkedlifedata.com/resource/pubmed/grant/AR50245,
http://linkedlifedata.com/resource/pubmed/grant/AR55434,
http://linkedlifedata.com/resource/pubmed/grant/F32 AR055434-03,
http://linkedlifedata.com/resource/pubmed/grant/P01 AR050245-07,
http://linkedlifedata.com/resource/pubmed/grant/R01 AG015768-12,
http://linkedlifedata.com/resource/pubmed/grant/R01 AR048182-05,
http://linkedlifedata.com/resource/pubmed/grant/R01 AR048852-04,
http://linkedlifedata.com/resource/pubmed/grant/R03 AR055659-01A1,
http://linkedlifedata.com/resource/pubmed/grant/R03 AR055659-02,
http://linkedlifedata.com/resource/pubmed/grant/R03 AR055659-03
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
1554-527X
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pubmed:author |
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pubmed:copyrightInfo |
Copyright © 2010 Orthopaedic Research Society.
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pubmed:issnType |
Electronic
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pubmed:volume |
29
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
501-10
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pubmed:dateRevised |
2011-9-22
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pubmed:meshHeading |
pubmed-meshheading:21337389-ADAM Proteins,
pubmed-meshheading:21337389-Animals,
pubmed-meshheading:21337389-Biological Markers,
pubmed-meshheading:21337389-Cartilage, Articular,
pubmed-meshheading:21337389-Cell Survival,
pubmed-meshheading:21337389-Chondrocytes,
pubmed-meshheading:21337389-Culture Media, Conditioned,
pubmed-meshheading:21337389-Disease Models, Animal,
pubmed-meshheading:21337389-Female,
pubmed-meshheading:21337389-Glycosaminoglycans,
pubmed-meshheading:21337389-Knee Injuries,
pubmed-meshheading:21337389-Matrix Metalloproteinases,
pubmed-meshheading:21337389-Mechanotransduction, Cellular,
pubmed-meshheading:21337389-Stifle,
pubmed-meshheading:21337389-Swine,
pubmed-meshheading:21337389-Weight-Bearing
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pubmed:year |
2011
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pubmed:articleTitle |
Cartilage viability and catabolism in the intact porcine knee following transarticular impact loading with and without articular fracture.
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pubmed:affiliation |
Division of Orthopaedic Surgery, Department of Surgery, Duke University Medical Center, Durham, North Carolina, USA.
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, N.I.H., Extramural
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